Maniatis T, Ptashne M, Backman K, Kield D, Flashman S, Jeffrey A, Maurer R
Cell. 1975 Jun;5(2):109-13. doi: 10.1016/0092-8674(75)90018-5.
Nucleotide sequences in two wild-type and six mutant operators in the DNA of phage lambda are compared. Strikingly similar 17 base pair units are found which we identify as the repressor binding sites. Each operator contains multiple repressor binding sites separated by A-T rich spacers. Elements of 2 fold rotational symmetry are present in each of the sites. Superimposed on each operator is an E. coli RNA polymerase recognition site (promoter). Similarities in the sequences of the two lambda promoters, a lac promoter, and an E. coli RNA polymerase recognition site in SV40 DNA are noted.
对噬菌体λ DNA 中的两个野生型和六个突变型操纵基因的核苷酸序列进行了比较。发现了惊人相似的 17 个碱基对单元，我们将其确定为阻遏物结合位点。每个操纵基因都包含多个由富含 A-T 的间隔区隔开的阻遏物结合位点。每个位点都存在二重旋转对称元件。每个操纵基因上都叠加有一个大肠杆菌 RNA 聚合酶识别位点（启动子）。还注意到了两个λ启动子、一个乳糖启动子以及 SV40 DNA 中的一个大肠杆菌 RNA 聚合酶识别位点的序列相似性。
