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噬菌体λ右向操纵基因的核苷酸序列。

Nucleotide sequence of the rightward operator of phage lambda.

作者信息

Maniatis T, Jeffrey A, Kleid D G

出版信息

Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8. doi: 10.1073/pnas.72.3.1184.

DOI:10.1073/pnas.72.3.1184
PMID:1055375
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC432491/
Abstract

The sequence of 72 base pairs of the rightward operator (O-R) of bacteriophage lambda is presented as determined with simple and rapid methods for direct DNA sequencing. The sequence of an operator mutant is also described. The methods are of general use in sequencing DNA fragments with unique 5' ends up to 50 base pairs in length. Previous experiments have shown that this operator contains multiple sites recognized by the lambda phage repressor. We believe we have identified three of these sites.

摘要

本文介绍了用简单快速的直接DNA测序方法测定的噬菌体λ右向操纵基因（O-R）72个碱基对的序列。还描述了一个操纵基因突变体的序列。这些方法普遍适用于对长度达50个碱基对且具有独特5'末端的DNA片段进行测序。先前的实验表明，该操纵基因含有多个被λ噬菌体阻遏物识别的位点。我们认为已确定了其中三个位点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b28/432491/d595896e14d3/pnas00046-0416-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b28/432491/9a8705cd424d/pnas00046-0415-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b28/432491/d595896e14d3/pnas00046-0416-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b28/432491/9a8705cd424d/pnas00046-0415-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6b28/432491/d595896e14d3/pnas00046-0416-a.jpg

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本文引用的文献

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DNA sequence analysis: a general, simple and rapid method for sequencing large oligodeoxyribonucleotide fragments by mapping.DNA序列分析：一种通过图谱对大的寡脱氧核糖核苷酸片段进行测序的通用、简单且快速的方法。
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A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.
烟草植株被 2 株互补的根癌农杆菌 octopine T 区突变体的双重感染。
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Transcription and translation of heat shock and normal proteins in seedlings and developing seeds of soybean exposed to a gradual temperature increase.在逐渐升温的条件下，大豆幼苗和发育种子中热激蛋白和正常蛋白的转录和翻译。
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Plant Mol Biol. 1988 Jul;11(4):389-99. doi: 10.1007/BF00039019.
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Mitochondrial DNA analyses of fertile and sterile maize plants derived from tissue culture with the texas male sterile cytoplasm.用德克萨斯细胞质雄性不育系组织培养的可育和不育玉米植株的线粒体 DNA 分析。
Theor Appl Genet. 1982 Sep;62(3):213-7. doi: 10.1007/BF00276241.
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4
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