Flashman S M
Mol Gen Genet. 1978 Oct 25;166(1):61-73. doi: 10.1007/BF00379730.
Oc mutations in the operators of bacteriophage lambda have been used to analyze the functional organization of the operators. In each operator, repressor binding sites 1 and 2, as identified biochemically, were found to be primarily responsible for the repressor affinity of the operators in vitro and for the repression of lytic functions in vivo. In addition, both sites were shown to be involved in the action of cro product at the operators. The data obtained have been used to estimate the repressor affinities of the individual binding sites. These affinities suggest that repressor bound at OR1 and OR2 interacts cooperatively. The results obtained support a model for repression of the early lambda operons where repressor bound at binding sites 1 and 2 interferes with RNA polymerase binding to the promotor sites.
噬菌体λ操纵子中的Oc突变已被用于分析操纵子的功能组织。在每个操纵子中，经生化鉴定的阻遏物结合位点1和2，被发现主要负责操纵子在体外对阻遏物的亲和力以及在体内对裂解功能的抑制。此外，这两个位点都显示参与了cro产物在操纵子上的作用。所获得的数据已被用于估计各个结合位点的阻遏物亲和力。这些亲和力表明结合在OR1和OR2上的阻遏物协同相互作用。所获得的结果支持了一个早期λ操纵子抑制模型，即结合在结合位点1和2上的阻遏物干扰RNA聚合酶与启动子位点的结合。
