Biundo J J, Lopez M, Kohler P F, Salvaggio J E
Clin Allergy. 1975 Jun;5(2):165-74. doi: 10.1111/j.1365-2222.1975.tb01849.x.
The technique of counterimmunoelectrophoresis (CIE) has been adapted for detection of serum precipitins to calf thymus (CT) DNA in patients with SLE, discoid LE, miscellaneous connective tissue and infectious diseases, and control populations. Of seventy-eight LE patients, 58% demonstrated anti-ss DNA precipitins, and 20% exhibited anti-ds DNA precipitins. Good correlation was noted between the presence of ss DNA precipitins and ss DNA binding values determined by the more sensitive ammonium sulphate precipitation assay. Depressed total serum haemolytic complement activity in CH50 mu/ml was noted in 64% of sera exhibiting ss DNA precipitins and 38% of those with negative ss DNA precipitins. There was a strong association, however, between ds DNA precipitins and depressed serum complement levels. Although less sensitive than primary binding assays, CIE can be used as a rapid and simple screening test for detection of circulating anti-native and denatured CT DNA precipitins. CT DNA serum precipitins are present in a significantly higher percentage of SLE patients when compared with other disease states and normal control populations.
对流免疫电泳（CIE）技术已被应用于检测系统性红斑狼疮（SLE）、盘状红斑狼疮（LE）、其他结缔组织病及传染病患者血清中针对小牛胸腺（CT）DNA的沉淀素，并与对照组进行比较。在78例LE患者中，58%显示抗单链DNA沉淀素，20%显示抗双链DNA沉淀素。单链DNA沉淀素的存在与通过更敏感的硫酸铵沉淀法测定的单链DNA结合值之间存在良好的相关性。在显示单链DNA沉淀素的血清中，64%的血清总溶血补体活性低于50单位/毫升，而在单链DNA沉淀素阴性的血清中这一比例为38%。然而，双链DNA沉淀素与血清补体水平降低之间存在很强的相关性。尽管CIE不如直接结合试验敏感，但它可作为一种快速简便的筛查试验，用于检测循环中抗天然和变性CT DNA沉淀素。与其他疾病状态和正常对照人群相比，SLE患者血清中CT DNA沉淀素的阳性率显著更高。
