Herzer P, Greif G, Lemmel E M
Klin Wochenschr. 1982 Feb 1;60(3):143-51. doi: 10.1007/BF01711278.
For measurement of antibodies of double-stranded DNA (ds DNA) the Crithidia luciliae immunofluorescence test (C1-IFT) was compared with the Farr precipitation technique in diagnosis and management of systemic lupus erythematosus (SLE). The C1-IFT proved to be less sensitive in SLE patients without clinical disease activity, but the sensitivity of the test in those with active disease was comparable to that of the Farr test. In 76.9% of the patients' sera identical test results were obtained by both assays. In some sera antibodies were detected only by the C1-IFT. Positive test results in patients with diseases other than SLE were found more often with the Farr test. Thus, The C1-IFT seemed to be more specific for diagnosis of SLE. Measurement of titers using the C1-IFT provides a simple method to survey SL E disease activity. It allows to analyze the immunoglobulin classes of the reacting antibodies and their complement fixation ability in vitro. All three major immunoglobulin classes (IgG, IgM, IgA) were found to be present in the sera with high antibody titers, complement fixation in the C1-IFT could predominantly be detected is sera with high antibody titers.
为了比较利什曼原虫免疫荧光试验（C1-IFT）与法尔沉淀技术在系统性红斑狼疮（SLE）诊断和管理中双链DNA（ds DNA）抗体测量方面的差异。结果表明，C1-IFT在无临床疾病活动的SLE患者中敏感性较低，但在有活动性疾病的患者中，该试验的敏感性与法尔试验相当。76.9%的患者血清通过两种检测方法获得了相同的检测结果。在一些血清中，仅通过C1-IFT检测到抗体。在非SLE疾病患者中，法尔试验更常出现阳性检测结果。因此，C1-IFT似乎对SLE的诊断更具特异性。使用C1-IFT测量滴度提供了一种简单的方法来监测SLE疾病活动。它允许在体外分析反应性抗体的免疫球蛋白类别及其补体固定能力。在高抗体滴度的血清中发现所有三种主要免疫球蛋白类别（IgG、IgM、IgA）均存在，C1-IFT中的补体固定主要在高抗体滴度的血清中检测到。
