Hurtubise P E, Coots M C, Jacob D J, Muhleman A F, Glueck H I
J Immunol. 1979 May;122(5):2119-21.
A monoclonal IgG4 (lambda) with inhibitory activity to human coagulation factor V was isolated from the serum of a patient with a fatal hemorrhagic diathesis by using a combination of DE-52 ion exchange chromatography and isoelectric focusing techniques. Using the criteria for defining a monoclonal immunoglobulin of restricted mobility on protein electrophoresis, immunoelectrophoresis, and isoelectric focusing, as well as neutralization with class, subclass, and light chain type antisera, we are the first to demonstrate a factor V inhibitor as a monoclonal IgG4 (lambda) detectable in serum or plasma.
通过结合DE-52离子交换色谱法和等电聚焦技术，从一名患有致命性出血素质的患者血清中分离出一种对人凝血因子V具有抑制活性的单克隆IgG4（λ）。利用蛋白质电泳、免疫电泳和等电聚焦中定义迁移受限的单克隆免疫球蛋白的标准，以及用类、亚类和轻链型抗血清进行中和，我们首次证明了一种可在血清或血浆中检测到的作为单克隆IgG4（λ）的因子V抑制剂。
