来自一名患者的两种单克隆蛋白IgG3（κ）和IgA1（λ）的双克隆起源。
A biclonal origin of two monoclonal proteins, IgG3(kappa) and IgA1(lambda), from a single patient.
作者信息
Ockhuizen T, Steen G, Muilerman H G, Smit J W, Wietzes P, Mandema E, Marrink J
出版信息
Immunology. 1979 Aug;37(4):863-8.
The cellulose acetate electrophoretic pattern of the serum from patient Sik disclosed two distinct peaks, representing two monoclonal proteins. On immunoelectrophoresis the two M-components were found to differ in heavy chain class as well as in light chain type, IgG3(kappa) and IgA1(lambda). Serum immunoglobulin levels remained relatively constant over a period of 7 years and no clinical symptoms of a malignant deterioration occurred. It was found that the isolated M-components did not share idiotypic antigenicity. Bone marrow cells synthesizing the monoclonal proteins were identified by means of the immunofluorescent technique using isotypic as well as idiotypic antisera. Two distinct monoclonal cell populations were observed, containing either the IgG3(kappa) or the IgA1(lambda) monoclonal protein. The alpha 1-chain belonged to the VHIII subgroup, whereas the gamma 3-chain was found to be blocked. Subsequent sequence determination showed the gamma 3-chain to belong to the VHIII subgroup. It was concluded that the two M-components in the serum of patient Sik resulted from two independent neoplastic transformations.
患者西克血清的醋酸纤维素电泳图谱显示出两个明显的峰，代表两种单克隆蛋白。免疫电泳显示这两种M成分在重链类别以及轻链类型上有所不同，分别为IgG3（κ）和IgA1（λ）。血清免疫球蛋白水平在7年期间保持相对稳定，未出现恶性恶化的临床症状。发现分离出的M成分不具有独特型抗原性。利用同型及独特型抗血清，通过免疫荧光技术鉴定出合成单克隆蛋白的骨髓细胞。观察到两个不同的单克隆细胞群体，分别含有IgG3（κ）或IgA1（λ）单克隆蛋白。α1链属于VHIII亚组，而γ3链被发现是封闭的。随后的序列测定表明γ3链属于VHIII亚组。得出的结论是，患者西克血清中的两种M成分是由两次独立的肿瘤转化产生的。
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