Miyagawa N, Morita K, Nakano H, Kawachi K
Department of Clinico-Laboratory Diagnostics, Nara Medical University, Kashihara.
Rinsho Byori. 1990 Nov;38(11):1306-10.
The serum from a 78-year-old male (SY) with abdominal aneurysm was shown to contain monoclonal protein (MP) of IgG. Immunoelectrophoresis (IEP) and immunofixation electrophoresis (IFE) of SY serum revealed MP reacted with both of anti kappa and anti lambda antisera. To see whether the monoclonal IgG molecule possesses both types of light chains, an attempt to separate MPs isolated by zone electrophoresis was made by affinity chromatography using anti kappa and anti lambda Sepharose 4 B conjugates. Immunodiffusion (ID) of these separated MPs showed that kappa type of MP (MP-kappa) was IgG1, while lambda type of MP (MP-lambda) was IgG3. For preparation of anti-idiotype antibodies (Aid) against MP-lambda, antisera against MP-lambda raised in rabbits by immunization with affinity-chromatographic purified MP-lambda was absorbed with solid phase normal human serum. ID and IFE of these MPs (original MP, MP-lambda and MP-kappa) revealed MP-kappa did not react with Aid, suggesting idiotype of MP-kappa was different from that of MP-lambda. These results indicate that two types of MPs derived from different B cell clones happen to have same mobility electrophoretically.
一名患有腹主动脉瘤的78岁男性（SY）的血清显示含有IgG单克隆蛋白（MP）。对SY血清进行免疫电泳（IEP）和免疫固定电泳（IFE）显示，MP与抗κ和抗λ抗血清均发生反应。为了确定单克隆IgG分子是否同时拥有两种类型的轻链，尝试通过使用抗κ和抗λ琼脂糖凝胶4B偶联物的亲和色谱法分离通过区带电泳分离的MP。对这些分离的MP进行免疫扩散（ID）显示，κ型MP（MP-κ）为IgG1，而λ型MP（MP-λ）为IgG3。为了制备针对MP-λ的抗独特型抗体（Aid），用亲和色谱纯化的MP-λ免疫兔子产生的抗MP-λ抗血清用固相正常人血清吸收。对这些MP（原始MP、MP-λ和MP-κ）进行ID和IFE显示，MP-κ不与Aid反应，表明MP-κ的独特型与MP-λ不同。这些结果表明，源自不同B细胞克隆的两种类型的MP在电泳中恰好具有相同的迁移率。
