一种作为大肠杆菌控制开关的反式作用RNA：DsrA通过形成可变结构来调节功能。
A trans-acting RNA as a control switch in Escherichia coli: DsrA modulates function by forming alternative structures.
作者信息
Lease R A, Belfort M
机构信息
Molecular Genetics Program, Wadsworth Center, New York State Department of Health, and School of Public Health, State University of New York at Albany, P.O. Box 22002, Albany, NY 12201-2002, USA.
出版信息
Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):9919-24. doi: 10.1073/pnas.170281497.
Abstract
DsrA is an 87-nucleotide regulatory RNA of Escherichia coli that acts in trans by RNA-RNA interactions with two different mRNAs, hns and rpoS. DsrA has opposite effects on these transcriptional regulators. H-NS levels decrease, whereas RpoS (final sigma(s)) levels increase. Here we show that DsrA enhances hns mRNA turnover yet stabilizes rpoS mRNA, either directly or via effects on translation. Computational and RNA footprinting approaches led to a refined structure for DsrA, and a model in which DsrA interacts with the hns mRNA start and stop codon regions to form a coaxial stack. Analogous bipartite interactions exist in eukaryotes, albeit with different regulatory consequences. In contrast, DsrA base pairs in discrete fashion with the rpoS RNA translational operator. Thus, different structural configurations for DsrA lead to opposite regulatory consequences for target RNAs.
摘要
DsrA是大肠杆菌中一种87个核苷酸的调控RNA，它通过与两种不同的mRNA（hns和rpoS）进行RNA - RNA相互作用而发挥反式作用。DsrA对这些转录调节因子有相反的作用。H - NS水平降低，而RpoS（最终的σ因子）水平升高。我们在此表明，DsrA增强hns mRNA的周转，但直接或通过对翻译的影响来稳定rpoS mRNA。计算和RNA足迹法得出了DsrA的精细结构，以及一个模型，其中DsrA与hns mRNA的起始和终止密码子区域相互作用形成同轴堆积。在真核生物中存在类似的二分相互作用，尽管具有不同的调控结果。相比之下，DsrA以离散方式与rpoS RNA翻译操纵子形成碱基对。因此，DsrA的不同结构构型导致对靶RNA产生相反的调控结果。
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