利用细胞表面突变体进行刀豆球蛋白A依赖性细菌凝集的简化检测方法。
Simplified assay for concanavalin A-dependent bacterial agglutination by using cell surface mutants.
作者信息
Maruyama H B, Arisawa M, Ono-Onitsuka M
出版信息
Infect Immun. 1975 Jun;11(6):1320-4. doi: 10.1128/iai.11.6.1320-1324.1975.
Abstract
Two cell surface mutants of Escherichia coli isolated through supersensitivity to sodium dodecyl sulfate were found to be agglutinated by concanavalin A in the intact rod form. By using these strains, the assay method for the concanavalin A-dependent agglutination was greatly simplified since neither spheroplast formation nor protease treatment is needed. This rod cell agglutination was characterized and applied for a semiquantitative determination of the amount of bound concanavalin A.
摘要
通过对十二烷基硫酸钠超敏感筛选出的两株大肠杆菌细胞表面突变体，在完整杆菌形态下可被伴刀豆球蛋白A凝集。利用这些菌株，伴刀豆球蛋白A依赖性凝集的检测方法得到了极大简化，因为既无需制备原生质体，也无需进行蛋白酶处理。对这种杆菌细胞凝集进行了表征，并将其应用于伴刀豆球蛋白A结合量的半定量测定。
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