大肠杆菌脂多糖缺陷菌株外膜中噬菌体λ受体蛋白损失的定量分析。
Quantitation of the loss of the bacteriophage lambda receptor protein from the outer membrane of lipopolysaccharide-deficient strains of Escherichia coli.
作者信息
Randall L L
出版信息
J Bacteriol. 1975 Jul;123(1):41-6. doi: 10.1128/jb.123.1.41-46.1975.
Abstract
The recpetor for the phage lambda, a protein component of the outer membrane, is present at decreased levels in strains of Escherichia coli that are deficient in lipopolysaccharide. Loss of the protein was quantitated both by an assay of the phage receptor function and by an assay of antiserum-blocking ability to detect inactive protein. The loss of protein was correlated with the loss of sugar residues and phosphage from the core region of the lipopolysaccharide. Implications for the importance of ionic interactions in the stabilization of the outer membrane are discussed.
摘要
噬菌体λ的受体是外膜的一种蛋白质成分，在缺乏脂多糖的大肠杆菌菌株中，其水平会降低。通过噬菌体受体功能测定和抗血清阻断能力测定来检测无活性蛋白质，从而对该蛋白质的损失进行定量。蛋白质的损失与脂多糖核心区域糖残基和磷酸的损失相关。文中讨论了离子相互作用在外膜稳定中的重要性。
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