Tomás J M, Kay W W
J Bacteriol. 1984 Sep;159(3):1047-52. doi: 10.1128/jb.159.3.1047-1052.1984.
The outer membrane of Escherichia coli was altered as a consequence of lysogeny by bacteriophages P1 and P1 cmts. The predominant change was a reduction in the size of lipopolysaccharide to a heptose-deficient form. P1 cmts lysogens were still sensitive to several bacteriophages but were resistant to lambda vir. Neither whole cells nor solubilized outer membranes from P1 cmts lysogens were able to inactivate lambda vir, and 32P-labeled lambda vir was unable to adsorb to P1 cmts lysogens. P1 cmts lysogens were also affected in maltose transport. The level of periplasmic maltose-binding protein was reduced somewhat, but there was no significant reduction in the level of the outer membrane lambda receptor (LamB). These membrane abnormalities were all corrected in strains cured of P1 cmts. It is suggested that P1 cmts affects lipopolysaccharide biosynthesis by a phage conversion mechanism and consequently the function of the lambda receptor.
由于噬菌体P1和P1 cmts的溶原作用，大肠杆菌的外膜发生了改变。主要变化是脂多糖的大小减小为缺乏庚糖的形式。P1 cmts溶原菌仍对几种噬菌体敏感，但对λvir具有抗性。来自P1 cmts溶原菌的全细胞和溶解的外膜均无法使λvir失活，并且32P标记的λvir无法吸附到P1 cmts溶原菌上。P1 cmts溶原菌在麦芽糖转运方面也受到影响。周质麦芽糖结合蛋白的水平有所降低，但外膜λ受体（LamB）的水平没有显著降低。在治愈了P1 cmts的菌株中，这些膜异常均得到了纠正。有人提出，P1 cmts通过噬菌体转化机制影响脂多糖的生物合成，从而影响λ受体的功能。
