Yagi N, Kamohara K, Itokawa Y
J Environ Pathol Toxicol. 1979 Mar-Apr;2(4):1119-25.
To clarify the effect of polychlorinated biphenyls (PCB), and dichlorodiphenyltrichloroethane (DDT) and other organochloride compounds on thiamine metabolism, rats were separated into six groups and placed on different admixed diets. Group 1 was fed a normal diet; group 2, a PCB-supplemented diet; group 3, a DDT-supplemented diet; group 4, a benzenehexachloride-supplemented (BHC) diet. After 50 days of these dietary regimens all animals were sacrificed and various studies were done on the excised tissues. In PCB-treated rats, thiamine levels in blood, liver and sciatic nerve decreased, transketolase activity in erythrocytes and liver decreased and TPP effect in erythrocytes and liver increased. In DDT-treated rats, thiamine levels in blood, brain and liver decreased, transketolase activity in brain and liver decreased and TPP effect in brain and liver increased. In other groups, no significant changes were observed regarding thiamine metabolism. Our findings provide direct evidence that changes occur in thiamine metabolism in PCB- and DDT-poisoned rats.
为阐明多氯联苯（PCB）、二氯二苯三氯乙烷（DDT）及其他有机氯化合物对硫胺素代谢的影响，将大鼠分为六组，给予不同的混合饲料。第1组喂以正常饲料；第2组喂以添加PCB的饲料；第3组喂以添加DDT的饲料；第4组喂以添加六氯环己烷（BHC）的饲料。在这些饮食方案实施50天后，处死所有动物，并对切除的组织进行各种研究。在PCB处理的大鼠中，血液、肝脏和坐骨神经中的硫胺素水平降低，红细胞和肝脏中的转酮醇酶活性降低，红细胞和肝脏中的TPP效应增加。在DDT处理的大鼠中，血液、大脑和肝脏中的硫胺素水平降低，大脑和肝脏中的转酮醇酶活性降低，大脑和肝脏中的TPP效应增加。在其他组中，未观察到硫胺素代谢有显著变化。我们的研究结果提供了直接证据，表明PCB和DDT中毒的大鼠硫胺素代谢发生了变化。
