一种用于选择带有表面免疫球蛋白的猪淋巴细胞的方法。
A method for the selection of pig lymphocytes bearing surface immunoglobulin.
作者信息
Symons D B, Loke R K
出版信息
J Immunol Methods. 1975 Jun;7(2-3):251-4. doi: 10.1016/0022-1759(75)90022-8.
PMID:1095655
Abstract
Coupling of IgG anti-immunoglobulin to polystyrene bottles with carbodiimide has been used to bind selectively pig lymphocytes previously treated with anti-immunoglobulin or anti-Ig class-specific fluorescent conjugates. Subsequent recovery of the bound cells produced populations with approximately 80% of cells stained for surface Ig and 90% viability.
摘要
利用碳二亚胺将抗免疫球蛋白IgG偶联到聚苯乙烯瓶上，以选择性结合先前用抗免疫球蛋白或抗Ig类特异性荧光共轭物处理过的猪淋巴细胞。随后回收结合的细胞，得到的细胞群体中约80%的细胞表面Ig被染色，细胞活力为90%。
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