Bräuniger S, Peters J, Borchers U, Kao M
Robert Koch Institute, Berlin, Germany.
Int J Hyg Environ Health. 2000 Mar;203(1):71-5. doi: 10.1078/S1438-4639(04)70010-3.
To supplement the results of thermal resistance of bovine parvovirus (Haden strain, BPV) published previously, we carried out assays at 60 degrees C (moist heat) to compare the thermal resistance of BPV with that of hepatitis B-virus (HBV). What we know about the resistance of HBV at a temperature of 60 degrees C is mainly based on data collected within the context of blood product pasteurization. The results suggest that at a temperature of 60 degrees C, BPV shows thermal resistance comparable to HBV. Thus, BPV--which is easier to handle--can be considered a good test virus to verify the efficacy of thermal disinfection techniques against HBV. BPV is very resistant against dry heat of 100 degrees C, the inactivation largely depending upon the residual moisture of the lyophilisate. Reducing the residual moisture from 2% to less than 1%, the exposure time has to be prolonged by ca. 2.5 times to achieve the same virucidal effect.
为补充之前发表的关于牛细小病毒（哈登株，BPV）热稳定性的结果，我们在60℃（湿热）条件下进行了试验，以比较BPV与乙型肝炎病毒（HBV）的热稳定性。我们所了解的HBV在60℃时的稳定性主要基于在血液制品巴氏消毒背景下收集的数据。结果表明，在60℃时，BPV的热稳定性与HBV相当。因此，易于处理的BPV可被视为验证针对HBV的热消毒技术效果的良好测试病毒。BPV对100℃的干热具有很强的抵抗力，其灭活很大程度上取决于冻干制品的残留水分。将残留水分从2%降至1%以下时，暴露时间必须延长约2.5倍才能达到相同的杀病毒效果。
