布拉迪斯拉发和因斯布鲁克分离出的医院内肠杆菌菌株中β-内酰胺耐药性的谱型及可转移性

Spectrum and transferability of beta-lactam resistance in hospital strains of Enterobacter isolated in Bratislava and Innsbruck.

作者信息

Bujdáková H, Klimáková J, Allerberger F, Moravcíková M, Bagová M, Hanzen J, Michálková-Papajová D, Dierich M P, Kettner M

机构信息

Department of Microbiology and Virology, Faculty of Natural Sciences, Comenius University, Bratislava, Slovak Republic.

出版信息

Int J Antimicrob Agents. 2000 Sep;16(1):31-6. doi: 10.1016/s0924-8579(00)00195-3.

DOI:10.1016/s0924-8579(00)00195-3

PMID:10957579

Abstract

The transferability and expression of beta-lactam resistance were compared in multiresistant clinical isolates of Enterobacter spp. collected from different hospitals in Bratislava, Slovakia (n = 15) and Innsbruck, Austria (n = 19) during 1996-1997. The strains from Bratislava were resistant to ampicillin, cefoxitin, cefotaxime, ceftazidime and ceftriaxone. All strains from Innsbruck were resistant to ampicillin and cefoxitin; 17 were also resistant to ceftazidime and aztreonam but the majority remained susceptible to cefotaxime and ceftriaxone. All strains were susceptible to cefepime and imipenem. The majority of the tested strains transferred resistance determinants to E. coli recipient by conjugation. Production of beta-lactamase including ESBL was the major mechanism of beta-lactam resistance. Large plasmids of 77-88 and 91 kb were confirmed in clinical isolates from Bratislava and Innsbruck.

摘要

1996 - 1997年期间，对从斯洛伐克布拉迪斯拉发（n = 15）和奥地利因斯布鲁克（n = 19）不同医院收集的多重耐药临床分离株肠杆菌属中β-内酰胺耐药性的转移性和表达进行了比较。来自布拉迪斯拉发的菌株对氨苄西林、头孢西丁、头孢噻肟、头孢他啶和头孢曲松耐药。来自因斯布鲁克的所有菌株对氨苄西林和头孢西丁耐药；17株对头孢他啶和氨曲南也耐药，但大多数对头孢噻肟和头孢曲松仍敏感。所有菌株对头孢吡肟和亚胺培南敏感。大多数测试菌株通过接合将耐药决定簇转移至大肠杆菌受体。包括超广谱β-内酰胺酶（ESBL）在内的β-内酰胺酶的产生是β-内酰胺耐药的主要机制。在来自布拉迪斯拉发和因斯布鲁克的临床分离株中证实存在77 - 88 kb和91 kb的大质粒。