Fonjungo P N, Mpoudi E N, Torimiro J N, Alemnji G A, Eno L T, Nkengasong J N, Gao F, Rayfield M, Folks T M, Pieniazek D, Lal R B
HIV/AIDS and Retrovirology Branch, Division of AIDS, STD and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
AIDS Res Hum Retroviruses. 2000 Sep 1;16(13):1319-24. doi: 10.1089/08892220050117087.
Phylogenetic analysis of the gp41 region of 123 HIV-1-seropositive specimens from Cameroon showed that 89 were subtype A (71% of these sequences were IbNg-like), 12 (10%) were subtype D, 11 (9%) were subtype G, 5 (4%; closely related to subtype F2) were subtype F, 1 was subtype H, 2 (1.6%) remained unclassifiable, while 3 were group O. Further analysis of the two unclassifiable specimens in gag(p24), pol(prot), and env (C2V3 or gp41) showed that one (98CM19) was a complex mosaic between subtype A in p24 and subtype J prot, and unclassifiable in env (C2V3 or gp41). The second, 98CM63, clustered distinctly from all known subtypes in p24, prot, C2V3, or gp41. 98CM63 clustered with a specimen from Cyprus and these two geographically and epidemiologically unlinked specimens, with their distinct clustering pattern, may represent a new subcluster of subtype A. In conclusion, these findings confirm the high HIV-1 genetic variability and further suggest the continuous appearance of new viral strains in this population.
对来自喀麦隆的123份HIV-1血清阳性样本的gp41区域进行系统发育分析显示，89份为A亚型（这些序列中有71%与IbNg样序列相似），12份（10%）为D亚型，11份（9%）为G亚型，5份（4%；与F2亚型密切相关）为F亚型，1份为H亚型，2份（1.6%）仍无法分类，另有3份为O组。对两份无法分类样本的gag（p24）、pol（蛋白酶）和env（C2V3或gp41）进行进一步分析表明，其中一份（98CM19）在p24中为A亚型，在蛋白酶中为J亚型，是一种复杂的镶嵌型，在env（C2V3或gp41）中无法分类。另一份样本98CM63在p24、蛋白酶、C2V3或gp41中与所有已知亚型明显聚类不同。98CM63与来自塞浦路斯的一份样本聚类在一起，这两份在地理和流行病学上没有关联的样本，具有独特的聚类模式，可能代表A亚型的一个新亚群。总之，这些发现证实了HIV-1的高遗传变异性，并进一步表明该人群中不断有新的病毒株出现。
