Ojeda S R, Wheaton J E, McCann S M
Neuroendocrinology. 1975;17(3):283-7. doi: 10.1159/000122364.
Prostaglandin E2 (PGE2) injected into the third ventricle (3rd V) of conscious ovariectomized rats bearing a permanent jugular cannula increased the percentage of plasma samples showing detectable immunoassayable LRF levels at 1, 3 and 5 min after injection. This percentage was small at 2 and 4 min. When plasma LRF and LH titers were measured in animals injected intraventicularly with PGE2 and decapitated 5 min later, both LRF and LH were significantly higher than control values of diluent-injected animals. These results indicate that PGE2 is acting on the hypothalamus to induce release of LRF sufficient in mangitude to be detected by measuring this neurohormone directly in peripheral plasma.
向有意识的、带有永久性颈静脉插管的去卵巢大鼠的第三脑室注射前列腺素E2（PGE2），在注射后1、3和5分钟时，显示可检测到免疫测定促性腺激素释放因子（LRF）水平的血浆样本百分比增加。在2和4分钟时该百分比很小。当对脑室注射PGE2并在5分钟后断头的动物测量血浆LRF和促黄体生成素（LH）滴度时，LRF和LH均显著高于注射稀释剂的对照动物的值。这些结果表明，PGE2作用于下丘脑以诱导释放足够量的LRF，从而能够通过直接在外周血浆中测量这种神经激素来检测到。
