Kikusui T, Aoyagi A, Kaneko T
Department of Animal Resource Science/Veterinary Medical Science, University of Tokyo, Japan.
Behav Neurosci. 2000 Aug;114(4):700-6.
This study investigated the relationship between spatial working memory and hippocampal long-term potentiation (LTP) using the allocentric place discrimination task (APDT) in rats, in which the selection accuracy is a good index for spatial working memory. Either the selective M1 muscarinic receptor antagonist pirenzepine (50 microg) or the choline uptake inhibitor hemicholinium-3 (5 microg) impaired APDT selection accuracy, but neither affected the induction of LTP in the hippocampal CA1 region in anesthetized rats. In contrast, the selective N-methyl-D-aspartate receptor antagonist D-amino-5-phosphonopentanoate (200 nmol) did not impair APDT selection accuracy but completely blocked hippocampal CA1 LTP. These results suggest that spatial working memory is independent of hippocampal CA1 LTP and that the central cholinergic system is involved in spatial working memory, but not through the modulation of hippocampal CAI LTP.
本研究使用大鼠的非中心位置辨别任务（APDT）来探究空间工作记忆与海马体长期增强效应（LTP）之间的关系，其中选择准确性是空间工作记忆的一个良好指标。选择性M1毒蕈碱受体拮抗剂哌仑西平（50微克）或胆碱摄取抑制剂半胱氨酸-3（5微克）均损害了APDT选择准确性，但两者均未影响麻醉大鼠海马体CA1区LTP的诱导。相比之下，选择性N-甲基-D-天冬氨酸受体拮抗剂D-氨基-5-磷酸戊酸（200纳摩尔）并未损害APDT选择准确性，但完全阻断了海马体CA1区的LTP。这些结果表明，空间工作记忆独立于海马体CA1区的LTP，并且中枢胆碱能系统参与空间工作记忆，但并非通过调节海马体CA1区的LTP来实现。
