Kaji A, Saheki T
Biochim Biophys Acta. 1975 Dec 18;410(2):354-60. doi: 10.1016/0005-2744(75)90237-5.
An arabinanase was purified from the culture fluid of Bacillus subtilis F-11. The process was as follows: salting out by (NH4)2SO4, repeated chromatography on hydroxy apatite and gel filtration on Sepharose-6B. The purified enzyme was demonstrated to be homogeneous by disc electrophoresis. The enzyme was found to be active on arabinan and 1,5-arabinan, but inactive on phenyl alpha-L-arabinofuranoside, p-nitrophenyl beta-D-galactopyranoside, arabinoxylan, gum arabic. The enzyme released arabinose, arabinobiose, arabinotriose and higher oligosaccharides during the course of hydrolysis of 1,5-arabinan. The end products were found to be arabinose and arabinobiose after 144 h of hydrolysis.
从枯草芽孢杆菌F-11的培养液中纯化出一种阿拉伯聚糖酶。纯化过程如下：用硫酸铵盐析，在羟基磷灰石上反复层析，以及在琼脂糖-6B上进行凝胶过滤。通过圆盘电泳证明纯化后的酶是均一的。该酶对阿拉伯聚糖和1,5-阿拉伯聚糖有活性，但对苯基α-L-阿拉伯呋喃糖苷、对硝基苯基β-D-吡喃半乳糖苷、阿拉伯木聚糖、阿拉伯胶无活性。在1,5-阿拉伯聚糖水解过程中，该酶释放出阿拉伯糖、阿拉伯二糖、阿拉伯三糖和更高的寡糖。水解144小时后，发现终产物为阿拉伯糖和阿拉伯二糖。
