枯草芽孢杆菌3-6来源的α-L-阿拉伯呋喃糖苷酶的纯化及某些性质
Purification and some properties of alpha-L-arabinofuranosidase from Bacillus subtilis 3-6.
作者信息
Kaneko S, Sano M, Kusakabe I
机构信息
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
出版信息
Appl Environ Microbiol. 1994 Sep;60(9):3425-8. doi: 10.1128/aem.60.9.3425-3428.1994.
alpha-L-Arabinofuranosidase (EC 3.2.1.55) was purified from culture supernatant of Bacillus subtilis 3-6. The enzyme had a molecular weight of 61,000 and displayed maximum activity at pH 7.0 and 60 degrees C. It released arabinose from O-alpha-L-arabinofuranosyl-(1-->3)-O-beta-D-xylopyranosyl-(1-->4)-D-x ylopyranos e (A1X2), O-beta-D-xylopyranosyl-(1-->4)-[O-alpha-L-arabinofuranosyl-(1-->3)]- O-beta-D-xylopyranosyl-(1-->4)-D-xylopyranose (A1X3), and arabinan, but not from O-beta-D-xylopyranosyl-(1-->2)-O-alpha-L- arabinofuranosyl-(1-->3)-O-beta-D-xylopyranosyl-(1-->4)-O-beta-D-xylopyr anosyl- (1-->4)-D-xylopyranose (A1X4), arabinoxylan, gum arabic, or arabinogalactan.
α-L-阿拉伯呋喃糖苷酶（EC 3.2.1.55）从枯草芽孢杆菌3-6的培养上清液中纯化得到。该酶分子量为61,000，在pH 7.0和60℃时表现出最大活性。它能从O-α-L-阿拉伯呋喃糖基-(1→3)-O-β-D-木吡喃糖基-(1→4)-D-木吡喃糖（A1X2）、O-β-D-木吡喃糖基-(1→4)-[O-α-L-阿拉伯呋喃糖基-(1→3)]-O-β-D-木吡喃糖基-(1→4)-D-木吡喃糖（A1X3）和阿拉伯聚糖中释放阿拉伯糖，但不能从O-β-D-木吡喃糖基-(1→2)-O-α-L-阿拉伯呋喃糖基-(1→3)-O-β-D-木吡喃糖基-(1→4)-O-β-D-木吡喃糖基-(1→4)-D-木吡喃糖（A1X4）、阿拉伯木聚糖、阿拉伯胶或阿拉伯半乳聚糖中释放。
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