衣原体中的高水平转录需要一个正向顺式作用DNA元件。
A positive cis-acting DNA element is required for high-level transcription in Chlamydia.
作者信息
Schaumburg C S, Tan M
机构信息
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92697-4025, USA.
出版信息
J Bacteriol. 2000 Sep;182(18):5167-71. doi: 10.1128/JB.182.18.5167-5171.2000.
Abstract
The spacer A/T region is a positive cis-acting DNA element that was identified in the Chlamydia trachomatis rRNA promoter region. We have now demonstrated that similar sequences in other chlamydial promoters are important for transcription. Substitution of candidate spacer A/T regions in four chlamydial promoters decreased transcription by partially purified C. trachomatis RNA polymerase in an in vitro transcription assay. Addition of a spacer A/T region to the dnaK promoter, which does not contain an identifiable spacer A/T region, increased transcription 16-fold. Transcription of Escherichia coli promoters by C. trachomatis RNA polymerase also appeared to be dependent on the spacer A/T region. However, the effect of the spacer A/T region on transcription by E. coli RNA polymerase was small. In summary, the spacer A/T region is a novel DNA element that is required for high-level transcription of many promoters by chlamydial RNA polymerase.
摘要
间隔区A/T区域是在沙眼衣原体rRNA启动子区域中鉴定出的一种正向顺式作用DNA元件。我们现已证明，其他衣原体启动子中的类似序列对转录很重要。在体外转录试验中，四个衣原体启动子中候选间隔区A/T区域的替换降低了部分纯化的沙眼衣原体RNA聚合酶的转录。向不包含可识别间隔区A/T区域的dnaK启动子中添加间隔区A/T区域，转录增加了16倍。沙眼衣原体RNA聚合酶对大肠杆菌启动子的转录似乎也依赖于间隔区A/T区域。然而，间隔区A/T区域对大肠杆菌RNA聚合酶转录的影响很小。总之，间隔区A/T区域是一种新型DNA元件，是衣原体RNA聚合酶对许多启动子进行高水平转录所必需的。
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