Miller D L, Martin N C, Pham H D, Donelson J E
J Biol Chem. 1979 Nov 25;254(22):11735-40.
Two restriction enzyme fragments containing yeast mitochondrial tRNA genes have been characterized by DNA sequence analysis. One of these fragments is 320 base pairs long and contains a tRNA Ser gene. The corresponding tRNA SER was isolated from yeast mitochondria and its nucleotide sequence also was determined. This mitochondrial tRNA is 90 nucleotides in length, has a G + C content of 38%, and has UGA as the anticodon. A portion of a 680-base-pair DNA fragment containing a tRNA Phe gene was also sequenced. The portion of this gene which codes for the mature tRNA is 75 base pairs in length, has a G + C content of 33%, and contains the anticodon GAA. Neither gene contains an intervening sequence or codes for the 3' CCA terminus. Both are surrounded by regions of more than 90% A + T. The significance of these sequences is discussed.
通过DNA序列分析对两个含有酵母线粒体tRNA基因的限制性酶切片段进行了表征。其中一个片段长320个碱基对，包含一个tRNA Ser基因。从酵母线粒体中分离出了相应的tRNA SER，并测定了其核苷酸序列。这种线粒体tRNA长度为90个核苷酸，G + C含量为38%，反密码子为UGA。还对一个含有tRNA Phe基因的680个碱基对DNA片段的一部分进行了测序。该基因编码成熟tRNA的部分长度为75个碱基对，G + C含量为33%，并含有反密码子GAA。这两个基因都不包含间隔序列，也不编码3' CCA末端。两者都被A + T含量超过90%的区域所包围。讨论了这些序列的意义。
