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利用羟胺裂解来产生核糖体蛋白S4的一个片段，该片段保留了特异性结合16S核糖体RNA的能力。

The use of hydroxylamine cleavage to produce a fragment of ribosomal protein S4 which retains the capacity to specifically bind 16S ribosomal RNA.

作者信息

Changchien L M, Craven G R

出版信息

Nucleic Acids Res. 1986 Mar 11;14(5):1957-66. doi: 10.1093/nar/14.5.1957.

DOI:10.1093/nar/14.5.1957
PMID:3515315
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC339635/
Abstract

In previous reports we have described the isolation of fragments of 30S ribosomal protein S4 using a number of different enzymatic and chemical cleavage techniques. These experiments were designed to determine the region of the protein responsible for 16S RNA recognition. We report here the isolation of two fragments produced by the hydroxylamine cleavage of the asparaginyl-glycyl peptide bond between positions 124 and 125. The purified fragments were chemically identified and tested for RNA binding capacity. The fragment consisting of residues 1-124 retains RNA binding activity and the fragment 125-203 is totally without RNA binding function. These results and previous results strongly suggest that the domain of protein S4 responsible for 16S RNA specific association is within the region consisting of residues 46-124.

摘要

在之前的报告中，我们描述了使用多种不同的酶切和化学裂解技术分离30S核糖体蛋白S4片段的过程。这些实验旨在确定该蛋白质中负责识别16S RNA的区域。我们在此报告通过羟胺裂解124位和125位之间的天冬酰胺基-甘氨酰肽键产生的两个片段的分离情况。对纯化后的片段进行了化学鉴定，并测试了其RNA结合能力。由1-124位残基组成的片段保留RNA结合活性，而125-203位的片段则完全没有RNA结合功能。这些结果以及之前的结果有力地表明，蛋白S4中负责与16S RNA特异性结合的结构域位于由46-124位残基组成的区域内。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b42d/339635/101f4c517af7/nar00274-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b42d/339635/9478b35d4d05/nar00274-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b42d/339635/101f4c517af7/nar00274-0039-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b42d/339635/9478b35d4d05/nar00274-0038-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b42d/339635/101f4c517af7/nar00274-0039-a.jpg

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Three-dimensional organization of the 30S ribosomal proteins from Escherichia coli. I. Preliminary classification of the proteins.大肠杆菌30S核糖体蛋白的三维结构。I. 蛋白质的初步分类。
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Nucleic Acids Res. 1987 Dec 23;15(24):10331-43. doi: 10.1093/nar/15.24.10331.
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Structure of rho factor: an RNA-binding domain and a separate region with strong similarity to proven ATP-binding domains.ρ因子的结构：一个RNA结合结构域和一个与已证实的ATP结合结构域有高度相似性的独立区域。
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Studies on the binding sites of protein S4 to 16S RNA in Escherichia coli ribosomes.关于蛋白质S4与大肠杆菌核糖体中16S RNA结合位点的研究。
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9
The function of the N-terminal region of ribosomal protein S4.核糖体蛋白S4 N端区域的功能。
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