Hurley M J, Mash D C, Jenner P
Neurology Department, University of Miami School of Medicine, 1501 NW 9th Avenue, FL, 33136, Miami, USA.
Neurosci Lett. 2000 Sep 8;291(1):54-8. doi: 10.1016/s0304-3940(00)01371-9.
The expression of the human adenosine A(2A) receptor was examined by reverse transcription polymerase chain reaction in post-mortem human brain tissue that was obtained from normal subjects and patients who died with Parkinson's disease. Adenosine A(2A) receptor mRNA was detected in both striatal (nucleus accumbens, caudate nucleus and putamen) and extrastriatal (globus pallidus and substantia nigra) brain regions. A significant decrease in the level of adenosine A(2A) receptor mRNA was found in the anterior and posterior caudate nucleus and anterior dorsal putamen, whereas a significant increase was observed in the substantia nigra pars reticulata of Parkinsonian brain when compared to age-matched controls. No change in adenosine A(2A) receptor mRNA levels was seen in any other brain region examined. This study demonstrates that A(2A) receptor mRNA expression is altered in the basal ganglia of patients who died with Parkinson's disease and who were receiving treatment with dopaminergic drugs. The adenosine A(2A) receptor appears subject to regulation by dopaminergic systems in human brain, though these data do not permit a distinction to be made between the effects of neuronal degeneration or drug treatment. The adenosine A(2A) receptor may therefore form a target for the treatment of basal ganglia disease.
通过逆转录聚合酶链反应，对取自正常受试者和帕金森病死亡患者的尸检人脑组织中人类腺苷A(2A)受体的表达进行了检测。在纹状体（伏隔核、尾状核和壳核）和纹状体以外（苍白球和黑质）的脑区均检测到腺苷A(2A)受体mRNA。与年龄匹配的对照组相比，在尾状核前后部和壳核前背部，腺苷A(2A)受体mRNA水平显著降低，而在帕金森病患者脑黑质网状部则显著升高。在所检测的任何其他脑区，腺苷A(2A)受体mRNA水平均未发现变化。本研究表明，在接受多巴胺能药物治疗的帕金森病死亡患者的基底节中，A(2A)受体mRNA表达发生了改变。腺苷A(2A)受体似乎受人类大脑中多巴胺能系统的调节，尽管这些数据无法区分神经元变性或药物治疗的影响。因此，腺苷A(2A)受体可能成为治疗基底节疾病的靶点。
