Jagannath M R, Vethanayagam R R, Reddy B S, Raman S, Rao C D
Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore.
Arch Virol. 2000;145(7):1339-57. doi: 10.1007/s007050070094.
In an epidemiological study of symptomatic human rotaviruses in Mysore, India during 1993 and 1994, isolates MP409 and MP480 were isolated from two children suffering from severe, acute dehydrating diarrhea. Both isolates exhibited 'long' RNA pattern and subgroup I specificity suggesting the likelihood of their animal origin. Both isolates did not react with monoclonal antibodies (MAbs) specific for serotypes G1 to G6 as well as G10. To determine the genetic origin of these isolates, complete nucleotide sequences of genes encoding the outer capsid proteins VP4 and VP7, nonstructural proteins NSP1 and NSP3 and viral enterotoxin protein NSP4 from MP409 and partial sequences of genes from MP480 were determined. Comparison of the 5' and 3' terminal sequences of 250 nucleotides revealed complete identity of the gene sequences in both strains suggesting that MP409 and MP480 are two different isolates of a single strain. Comparison of the nucleotide and deduced amino acid sequences of VP4, VP7, NSP1 and NSP3 of MP409 with published sequences of strains belonging to different serotypes revealed that both outer capsid proteins VP4 and VP7 and NSPI are highly related to the respective proteins from the P6[1], G8 type bovine rotavirus A5 isolated from a calf with diarrhoea in Thailand and that the NSP3 is highly homologous to that of bovine rotaviruses. The NSP4 protein showed greatest sequence identity with NSP4s belonging to the KUN genetic group to which NSP4s from human G2 type strains and bovine rotaviruses belong. MP409 and MP480 likely signify interspecies transmission of P6[1], G8 type strains from cattle to humans and represent the first P6[1] type rotaviruses isolated in humans. These and our previous studies on the asymptomatic neonatal strain 1321 are of evolutionary and epidemiological significance in the context of close association of majority of the Indian population with cattle.
在1993年和1994年于印度迈索尔开展的一项关于有症状人类轮状病毒的流行病学研究中，从两名患有严重急性脱水腹泻的儿童体内分离出了MP409和MP480毒株。这两种毒株均呈现“长”RNA模式和I亚组特异性，表明它们可能源自动物。这两种毒株均不与针对G1至G6以及G10血清型的单克隆抗体（MAb）发生反应。为确定这些毒株的基因起源，测定了MP409中编码外衣壳蛋白VP4和VP7、非结构蛋白NSP1和NSP3以及病毒肠毒素蛋白NSP4的基因的完整核苷酸序列，以及MP480基因的部分序列。对250个核苷酸的5'和3'末端序列进行比较后发现，两种毒株的基因序列完全相同，这表明MP409和MP480是单一毒株的两个不同分离株。将MP409的VP4、VP7、NSP1和NSP3的核苷酸及推导的氨基酸序列与不同血清型毒株的已发表序列进行比较后发现，两种外衣壳蛋白VP4和VP7以及NSPI与从泰国一头腹泻小牛分离出的P6[1]、G8型牛轮状病毒A5的相应蛋白高度相关，且NSP3与牛轮状病毒的NSP3高度同源。NSP4蛋白与属于KUN基因组的NSP4具有最高的序列同一性，人类G2型毒株和牛轮状病毒的NSP4均属于该基因组。MP409和MP480可能意味着P6[1]、G8型毒株从牛到人的种间传播，并且代表了在人类中分离出的首个P6[1]型轮状病毒。鉴于印度大多数人口与牛密切相关，这些研究以及我们之前对无症状新生儿毒株1321的研究具有进化和流行病学意义。
