Sircar Shubhankar, Malik Yashpal Singh, Kumar Prashant, Ansari Mohd Ikram, Bhat Sudipta, Shanmuganathan S, Kattoor Jobin Jose, Vinodhkumar O R, Rishi Narayan, Touil Nadia, Ghosh Souvik, Bányai Krisztián, Dhama Kuldeep
Division of Biological Standardization, Indian Council of Agricultural Research-Indian Veterinary Research Institute, Bareilly, India.
Amity Institute of Virology and Immunology, J-3 Block, Amity University, Noida, India.
Front Vet Sci. 2021 Jan 27;7:606661. doi: 10.3389/fvets.2020.606661. eCollection 2020.
The surveillance studies for the presence of caprine rotavirus A (RVA) are limited in India, and the data for the whole-genome analysis of the caprine RVA is not available. This study describes the whole-genome-based analysis of a caprine rotavirus A strain, RVA/Goat-wt/IND/K-98/2015, from a goat kid in India. The genomic analysis revealed that the caprine RVA strain K-98, possess artiodactyl-like and DS-1 human-like genome constellation G8P[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3. The three structural genes (VP2, VP4, and VP7) were close to caprine host having nucleotide-based identity range between 97.5 and 98.9%. Apart from them, other gene segments showed similarity with either bovine or human like genes, ultimately pointing toward a common evolutionary origin having an artiodactyl-type backbone of strain K-98. Phylogenetically, the various genes of the current study isolate also clustered inside clades comprising Human-Bovine-Caprine isolates from worldwide. The current findings add to the knowledge on caprine rotaviruses and might play a substantial role in designing future vaccines or different alternative strategies combating such infections having public health significance. To the best of our knowledge, this is the first report on the whole-genome characterization of a caprine RVA G8P[1] strain from India. Concerning the complex nature of the K-98 genome, whole-genome analyses of more numbers of RVA strains from different parts of the country are needed to comprehend the genomic nature and genetic diversity among caprine RVA.
在印度，关于山羊轮状病毒A（RVA）存在情况的监测研究有限，且尚无山羊RVA全基因组分析的数据。本研究描述了对一株来自印度山羊幼崽的山羊轮状病毒A毒株RVA/Goat-wt/IND/K-98/2015进行的全基因组分析。基因组分析显示，山羊RVA毒株K-98具有偶蹄类样和DS-1人类样基因组组合G8P[1]-I2-R2-C2-M2-A3-N2-T6-E2-H3。三个结构基因（VP2、VP4和VP7）与山羊宿主相近，基于核苷酸的同一性范围在97.5%至98.9%之间。除此之外，其他基因片段与牛或人类样基因显示出相似性，最终表明毒株K-98具有偶蹄类型主干的共同进化起源。在系统发育上，本研究分离株的各种基因也聚集在包含来自世界各地的人-牛-山羊分离株的进化枝内。目前的研究结果增加了对山羊轮状病毒的认识，可能在设计未来疫苗或对抗此类具有公共卫生意义感染的不同替代策略中发挥重要作用。据我们所知，这是关于来自印度的山羊RVA G8P[1]毒株全基因组特征的首次报告。鉴于K-98基因组的复杂性，需要对来自该国不同地区的更多RVA毒株进行全基因组分析，以了解山羊RVA的基因组性质和遗传多样性。
