Saurabh Sharad, Sircar Shubhankar, Kattoor Jobin J, Ghosh Souvik, Kobayashi Nobumichi, Banyai Krisztian, VinodhKumar Obli R, De Ujjwal K, Sahoo Nihar R, Dhama Kuldeep, Malik Yashpal S
Indian Council of Agricultural Research-Indian Veterinary Research Institute, Uttar Pradesh 243122, India.
Department of Biomedical Sciences, One Health Center for Zoonoses and Tropical Veterinary Medicine, Ross University School of Veterinary Medicine, P.O. Box 334, Basseterre, Federation of Saint Kitts and Nevis.
J Vet Sci. 2018 Jan 31;19(1):35-43. doi: 10.4142/jvs.2018.19.1.35.
Rotavirus (RV)-infected piglets are presumed to be latent sources of heterologous RV infection in humans and other animals. In RVs, non-structural protein 4 (NSP4) is the major virulence factor with pleiotropic properties. In this study, we analyzed the gene from porcine RVs isolated from diarrheic and non-diarrheic cases at different levels of protein folding to explore correlations to diarrhea-inducing capabilities and evolution of in the porcine population. Full-length genes were amplified, cloned, sequenced, and then analyzed for antigenic epitopes, RotaC classification, homology, genetic relationship, modeling of NSP4 protein, and prediction of post-translational modification. RV presence was observed in both diarrheic and non-diarrheic piglets. All genes possessed the E1 genotype. Comparison of primary, secondary, and tertiary structure and the prediction of post-translational modifications of NSP4 from diarrheic and non-diarrheic piglets revealed no apparent differences. Sequence analysis indicated that genes have a multi-phyletic evolutionary origin and exhibit species independent genetic diversity. The results emphasize the evolution of the E9 genotype from the E1 genotype and suggest that the diarrhea-inducing capability of porcine RVs may not be exclusively linked to its enterotoxin gene.
轮状病毒（RV）感染的仔猪被认为是人类和其他动物中异源RV感染的潜在来源。在RV中，非结构蛋白4（NSP4）是具有多效性的主要毒力因子。在本研究中，我们从蛋白质折叠的不同水平分析了从腹泻和非腹泻病例中分离的猪RV的基因，以探索与猪群体中腹泻诱导能力和基因进化的相关性。扩增、克隆、测序全长基因，然后分析其抗原表位、RotaC分类、同源性、遗传关系、NSP4蛋白建模以及翻译后修饰预测。在腹泻和非腹泻仔猪中均观察到RV的存在。所有基因均具有E1基因型。腹泻和非腹泻仔猪NSP4的一级、二级和三级结构比较以及翻译后修饰预测均未发现明显差异。序列分析表明，基因具有多系进化起源，并表现出物种独立的遗传多样性。结果强调了E9基因型从E1基因型的进化，并表明猪RV的腹泻诱导能力可能并非仅与其肠毒素基因相关。
