Marksteiner J, Lechner T, Kaufmann W A, Gurka P, Humpel C, Nowakowski C, Maier H, Jellinger K A
Department of Psychiatry, University of Innsbruck, Austria.
Acta Neuropathol. 2000 Aug;100(2):205-12. doi: 10.1007/s004010000239.
Synapse loss is crucially involved in cognitive decline in Alzheimer's disease (AD). This study was performed to investigate the distribution and density of chromogranin B-like immunoreactivity in the hippocampus of control compared to AD brain. Chromogranin B is a large precursor molecule found in large dense-core vesicles. For immunocytochemistry we used an antiserum raised against a synthetic peptide (PE- 11) present in the chromogranin B molecule. Chromogranin B-like immunoreactivity was concentrated in the terminal field of mossy fibers, the inner molecular layer of the dentate gyrus and in layer II of the entorhinal cortex. In AD, chromogranin B was detected in neuritic plaques. The density of chromogranin B-like immunoreactivity was significantly reduced in the inner molecular layer of the dentate gyrus and in layers II, III and V of the entorhinal cortex in AD brains. The present study demonstrates that chromogranin B is a marker for human hippocampal pathways. It is particularly suitable for studying nerve fibers terminating at the inner molecular layer of the dentate gyrus. It is present in neuritic plaques, and its density is reduced in a layer-specific manner.
突触丢失在阿尔茨海默病（AD）的认知衰退中起着关键作用。本研究旨在调查与AD脑相比，对照脑海马中嗜铬粒蛋白B样免疫反应性的分布和密度。嗜铬粒蛋白B是一种存在于大致密核心囊泡中的大前体分子。对于免疫细胞化学，我们使用了针对嗜铬粒蛋白B分子中存在的合成肽（PE-11）产生的抗血清。嗜铬粒蛋白B样免疫反应性集中在苔藓纤维的终末场、齿状回的内分子层和内嗅皮质的II层。在AD中，在神经炎性斑块中检测到嗜铬粒蛋白B。在AD脑的齿状回内分子层以及内嗅皮质的II、III和V层中，嗜铬粒蛋白B样免疫反应性的密度显著降低。本研究表明，嗜铬粒蛋白B是人类海马通路的标志物。它特别适合于研究终止于齿状回内分子层的神经纤维。它存在于神经炎性斑块中，并且其密度以层特异性方式降低。
