Kaufmann W A, Barnas U, Humpel C, Nowakowski K, DeCol C, Gurka P, Ransmayr G, Hinterhuber H, Winkler H, Marksteiner J
Clinic of Psychiatry, Innsbruck, Austria.
Eur J Neurosci. 1998 Mar;10(3):1084-94. doi: 10.1046/j.1460-9568.1998.00121.x.
Secretoneurin is a recently described peptide derived by endoproteolytic processing from secretogranin II, previously named chromogranin C. In this study, we have investigated the distribution of secretoneurin-like immunoreactivity in the human hippocampus in controls and in Alzheimer's disease patients, and compared the staining pattern to that of calretinin. Secretoneurin-like immunoreactivity is present throughout the hippocampal formation. At the border of the dentate molecular layer and the granule cell layer, a band of dense secretoneurin immunostaining appeared. In this part, as in the area of the CA2 sector, the high density of secretoneurin-immunoreactivity coincided with calretinin-like immunoreactivity. The mossy fibre system displayed a moderate density of secretoneurin-immunoreactivity. In the entorhinal cortex, a particularly high density of secretoneurin-immunoreactivity was observed. The density of secretoneurin-like immunoreactivity was significantly reduced in the innermost part of the molecular layer and in the outer molecular layer of the dentate gyrus in Alzheimer's disease. For calretinin-like immunoreactivity, a less pronounced decrease was found in the innermost part of the molecular layer. About 40-60% of neuritic plaques were secretoneurin-immunopositive. This study shows that secretoneurin is distinctly distributed in the human hippocampus and that significant changes of secretoneurin-like immunoreactivity occur in Alzheimer's disease, reflecting synaptic loss.
促分泌素原神经肽是一种最近发现的肽，它是由分泌粒蛋白II经内蛋白水解加工产生的，分泌粒蛋白II之前被称为嗜铬粒蛋白C。在本研究中，我们调查了促分泌素原神经肽样免疫反应性在对照组和阿尔茨海默病患者的人海马体中的分布，并将染色模式与钙视网膜蛋白的染色模式进行了比较。促分泌素原神经肽样免疫反应性存在于整个海马结构中。在齿状分子层和颗粒细胞层的边界处，出现了一条密集的促分泌素原神经肽免疫染色带。在这部分区域，如同在CA2区一样，促分泌素原神经肽免疫反应性的高密度与钙视网膜蛋白样免疫反应性相吻合。苔藓纤维系统显示出中等密度的促分泌素原神经肽免疫反应性。在内嗅皮质中，观察到促分泌素原神经肽免疫反应性的密度特别高。在阿尔茨海默病中，齿状回分子层最内层和外分子层中促分泌素原神经肽样免疫反应性的密度显著降低。对于钙视网膜蛋白样免疫反应性，在分子层最内层发现的降低不太明显。约40%-60%的神经炎性斑块促分泌素原神经肽免疫呈阳性。这项研究表明，促分泌素原神经肽在人海马体中分布明显，并且在阿尔茨海默病中促分泌素原神经肽样免疫反应性发生显著变化，反映了突触丧失。
