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Studies on the noncooperative binding of the Escherichia coli DNA unwinding protein to single-stranded nucleic acids.

作者信息

Ruyechan W T, Wetmur J G

出版信息

Biochemistry. 1976 Nov 16;15(23):5057-64. doi: 10.1021/bi00668a017.

DOI:10.1021/bi00668a017
PMID:10965
Abstract

The noncooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA oligomers has been studied by means of equilibrium dialysis. Dialyses were performed under a number of solution and temperature conditions using oligomers of varying length and base compositions. The results of these studies, which include a Scatchard analysis of the binding, have allowed us to propose a model for the cooperative binding of the protein to single-stranded DNA. The results of experiments dealing with the interaction of the protein with single-stranded RNA are also presented.

摘要

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