• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大肠杆菌DNA解旋蛋白与单链DNA协同结合的研究。

Studies on the cooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA.

作者信息

Ruyechan W T, Wetmur J G

出版信息

Biochemistry. 1975 Dec 16;14(25):5529-34. doi: 10.1021/bi00696a023.

DOI:10.1021/bi00696a023
PMID:1103970
Abstract

The cooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA has been studied by electron microscopy. Analysis of the electron microscopic data by means of a simple statistical mechanical model has yielded a value of 3.8-7.6 X 10(10) l./mol for the cooperative binding constant in 0.15 M NaCl. Studied under elevated salt conditions have shown that the average DNA protein complex length is 50% of the length found at 0.04 or 0.15 M NaCl.

摘要

通过电子显微镜研究了大肠杆菌DNA解旋蛋白与单链DNA的协同结合。借助一个简单的统计力学模型对电子显微镜数据进行分析，得出在0.15M NaCl中协同结合常数的值为3.8 - 7.6×10¹⁰ l/mol。在高盐条件下的研究表明，DNA - 蛋白质复合物的平均长度是在0.04M或0.15M NaCl中所发现长度的50%。

相似文献

1
Studies on the cooperative binding of the Escherichia coli DNA unwinding protein to single-stranded DNA.大肠杆菌DNA解旋蛋白与单链DNA协同结合的研究。
Biochemistry. 1975 Dec 16;14(25):5529-34. doi: 10.1021/bi00696a023.
2
Studies on the noncooperative binding of the Escherichia coli DNA unwinding protein to single-stranded nucleic acids.
Biochemistry. 1976 Nov 16;15(23):5057-64. doi: 10.1021/bi00668a017.
3
Knotted single-stranded DNA rings: a novel topological isomer of circular single-stranded DNA formed by treatment with Escherichia coli omega protein.扭结单链DNA环：一种通过用大肠杆菌ω蛋白处理形成的新型环状单链DNA拓扑异构体。
J Mol Biol. 1976 Sep 15;106(2):439-52. doi: 10.1016/0022-2836(76)90095-4.
4
Interaction of DNA with DNA binding proteins. II. Displacement of Escherichia coli DNA unwinding protein and the condensed structure of DNA complexed with protein HD.DNA与DNA结合蛋白的相互作用。II. 大肠杆菌DNA解旋蛋白的置换以及与蛋白HD复合的DNA的凝聚结构
Biochim Biophys Acta. 1977 Feb 16;474(4):629-38. doi: 10.1016/0005-2787(77)90082-x.
5
The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication.大肠杆菌的脱氧核糖核酸解旋蛋白。复制过程中的性质与功能。
J Biol Chem. 1975 Mar 25;250(6):1972-80.
6
Binding of Escherichia coli primary replicative helicase DnaB protein to single-stranded DNA. Long-range allosteric conformational changes within the protein hexamer.大肠杆菌主要复制解旋酶DnaB蛋白与单链DNA的结合。蛋白质六聚体内的长程变构构象变化。
Biochemistry. 1996 Feb 20;35(7):2129-45. doi: 10.1021/bi952345d.
7
Equilibrium binding of Escherichia coli single-strand binding protein to single-stranded nucleic acids in the (SSB)65 binding mode. Cation and anion effects and polynucleotide specificity.大肠杆菌单链结合蛋白以(SSB)65结合模式与单链核酸的平衡结合。阳离子和阴离子效应以及多核苷酸特异性。
Biochemistry. 1988 Jan 12;27(1):456-71. doi: 10.1021/bi00401a067.
8
A highly salt-dependent enthalpy change for Escherichia coli SSB protein-nucleic acid binding due to ion-protein interactions.由于离子与蛋白质的相互作用，大肠杆菌单链结合蛋白（SSB）与核酸结合存在高度依赖盐的焓变。
Biochemistry. 1996 Apr 23;35(16):5272-9. doi: 10.1021/bi9527606.
9
Quantitative analysis of binding of single-stranded DNA by Escherichia coli DnaB helicase and the DnaB x DnaC complex.大肠杆菌DnaB解旋酶及DnaB x DnaC复合物与单链DNA结合的定量分析。
Biochemistry. 2006 Sep 26;45(38):11505-13. doi: 10.1021/bi060118d.
10
A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases.从大肠杆菌中分离出的一种DNA解旋蛋白：它与DNA及DNA聚合酶的相互作用。
Proc Natl Acad Sci U S A. 1972 Dec;69(12):3537-41. doi: 10.1073/pnas.69.12.3537.

引用本文的文献

1
Structural basis for cooperative ssDNA binding by bacteriophage protein filament P12.噬菌体蛋白丝P12协同结合单链DNA的结构基础
Nucleic Acids Res. 2025 Feb 27;53(5). doi: 10.1093/nar/gkaf132.
2
Structural transitions during the cooperative assembly of baculovirus single-stranded DNA-binding protein on ssDNA.杆状病毒单链 DNA 结合蛋白在 ssDNA 上协同组装过程中的结构转变。
Nucleic Acids Res. 2022 Dec 9;50(22):13100-13113. doi: 10.1093/nar/gkac1142.
3
OB-fold Families of Genome Guardians: A Universal Theme Constructed From the Small β-barrel Building Block.
基因组守护者的 OB 折叠家族：由小β桶结构单元构建的通用主题。
Front Mol Biosci. 2022 Feb 11;9:784451. doi: 10.3389/fmolb.2022.784451. eCollection 2022.
4
The mechanism of action of the SSB interactome reveals it is the first OB-fold family of genome guardians in prokaryotes.SSB 相互作用组的作用机制揭示了它是原核生物中第一个 OB 折叠家族的基因组守护者。
Protein Sci. 2021 Sep;30(9):1757-1775. doi: 10.1002/pro.4140. Epub 2021 Jun 14.
5
Multiprotein E. coli SSB-ssDNA complex shows both stable binding and rapid dissociation due to interprotein interactions.多蛋白大肠杆菌 SSB-ssDNA 复合物由于蛋白间相互作用表现出稳定的结合和快速的解离。
Nucleic Acids Res. 2021 Feb 22;49(3):1532-1549. doi: 10.1093/nar/gkaa1267.
6
Regulation of Nearest-Neighbor Cooperative Binding of E. coli SSB Protein to DNA.大肠杆菌 SSB 蛋白与 DNA 的近邻协同结合的调控。
Biophys J. 2019 Dec 3;117(11):2120-2140. doi: 10.1016/j.bpj.2019.09.047. Epub 2019 Oct 28.
7
Structural Mechanisms of Cooperative DNA Binding by Bacterial Single-Stranded DNA-Binding Proteins.细菌单链 DNA 结合蛋白协同 DNA 结合的结构机制。
J Mol Biol. 2019 Jan 18;431(2):178-195. doi: 10.1016/j.jmb.2018.11.019. Epub 2018 Nov 22.
8
Glutamate promotes SSB protein-protein Interactions via intrinsically disordered regions.谷氨酸通过内在无序区域促进单链结合蛋白（SSB）的蛋白质-蛋白质相互作用。
J Mol Biol. 2017 Sep 1;429(18):2790-2801. doi: 10.1016/j.jmb.2017.07.021. Epub 2017 Aug 3.
9
Structural dynamics of E. coli single-stranded DNA binding protein reveal DNA wrapping and unwrapping pathways.大肠杆菌单链DNA结合蛋白的结构动力学揭示了DNA包裹和解开途径。
Elife. 2015 Aug 25;4:e08193. doi: 10.7554/eLife.08193.
10
Intrinsically disordered C-terminal tails of E. coli single-stranded DNA binding protein regulate cooperative binding to single-stranded DNA.大肠杆菌单链DNA结合蛋白的内在无序C末端尾巴调节与单链DNA的协同结合。
J Mol Biol. 2015 Feb 27;427(4):763-774. doi: 10.1016/j.jmb.2014.12.020. Epub 2015 Jan 3.