Xia Jian-Chuan, Weng De-Sheng, Li Jin-Tian, Qin Hai-De, Mai Shi-Juan, Feng Bing-Jian, Fan Qin, Feng Qi-Sheng, Huang Li-Xi, Yu Xing-Juan, Pan Zhi-Zhong, Li Yong-Qiang, Wang Qi-Jing, Zhan You-Qing, Chen Shi-Ping, He Jia, Huang Wen-lin, Wu Pei-Hong, Zeng Yi-Xing
Research Section of Oncopathology, State Key Laboratory of Oncology in Southern China, Cancer Center, Zhongshan University, 651 Dong-feng Road East, Guangzhou 510060, China.
Cancer Genet Cytogenet. 2006 Apr 15;166(2):166-72. doi: 10.1016/j.cancergencyto.2005.11.005.
Gastric carcinoma is one of the most common malignancies in Asia. Although the allelic deletion of 7q has been reportedly associated with primary gastric carcinoma tumorigenesis, no predisposing genes in this region have been identified so far. Here, we report the results of genotype and loss of heterozygosity (LOH) analysis on 7q in this tumor. A panel of nine microsatellite markers distributed over the whole chromosome 7q was used for genotyping primary gastric carcinomas. Of 72 primary tumors LOH of D7S486 occurred in 24.0% (12/50) of cases. Fine mapping with 12 additional markers flanking D7S486 resulted in LOH of 30.36% (17/56) and defined one minimal deleted region in primary gastric carcinomas, a 90-kilobase region bounded by D7S2543 and D7S486 at 7q31.2. The allelic deletion correlates statistically with clinicopathologic variables. Our data suggest a possible link between putative tumor suppressor genes and gastric carcinoma in the 7q31 region.
胃癌是亚洲最常见的恶性肿瘤之一。尽管据报道7q等位基因缺失与原发性胃癌的肿瘤发生有关，但迄今为止该区域尚未发现易感基因。在此，我们报告了对该肿瘤7q进行基因分型和杂合性缺失（LOH）分析的结果。一组分布于整个7号染色体长臂的9个微卫星标记用于原发性胃癌的基因分型。在72例原发性肿瘤中，D7S486的LOH在24.0%（12/50）的病例中出现。用位于D7S486两侧的另外12个标记进行精细定位，结果显示LOH为30.36%（17/56），并在原发性胃癌中确定了一个最小缺失区域，即7q31.2处由D7S2543和D7S486界定的一个90千碱基区域。等位基因缺失与临床病理变量具有统计学相关性。我们的数据表明，7q31区域中假定的肿瘤抑制基因与胃癌之间可能存在联系。
