Ezaki T, Yanagisawa A, Ohta K, Aiso S, Watanabe M, Hibi T, Kato Y, Nakajima T, Ariyama T, Inazawa J, Nakamura Y, Horii A
Department of Biochemistry, Cancer Institute, Tokyo, Japan.
Br J Cancer. 1996 Feb;73(4):424-8. doi: 10.1038/bjc.1996.76.
To define the region on the short arm of chromosome 1 that is thought to include one or more tumour-suppressor genes for gastric cancers, we carried out loss of heterozygosity (LOH) studies in 26 gastric adenocarcinomas, using three restriction fragment length polymorphism (RFLP) markers and nine microsatellite markers. All tumours were informative with at least one locus; three revealed replication errors (RERs) at multiple microsatellite loci, and interstitial or telomeric allelic deletions were observed in 12 cases. Deletion mapping of these tumours defined a commonly deleted region between two loci, D1S201 and D1S197, that are 13 cM apart. As two loci within the commonly deleted region, D1S57 (pYNZ2) and D1S62 (pTHI54), were mapped respectively to 1p35 and 1p34.3 by fluorescence in situ hybridisation, we conclude that a locus likely to contain a tumour-suppressor gene for gastric cancer is located within a 13 cM region encompassing two chromosomal bands.
为了确定1号染色体短臂上被认为包含一个或多个胃癌肿瘤抑制基因的区域，我们使用三个限制性片段长度多态性（RFLP）标记和九个微卫星标记，对26例胃腺癌进行了杂合性缺失（LOH）研究。所有肿瘤至少在一个位点具有信息性；三个在多个微卫星位点显示出复制错误（RER），并且在12例中观察到间质或端粒等位基因缺失。这些肿瘤的缺失图谱确定了两个相距13厘摩的位点D1S201和D1S197之间的一个常见缺失区域。由于通过荧光原位杂交将常见缺失区域内的两个位点D1S57（pYNZ2）和D1S62（pTHI54）分别定位到1p35和1p34.3，我们得出结论，一个可能包含胃癌肿瘤抑制基因的位点位于一个包含两条染色体带的13厘摩区域内。
