Fergusson K M, Saint C P
The Cooperative Research Centre for Water Quality and Treatment, Australian Water Quality Centre, SA Water Corporation, Salisbury, South Australia 5108, Australia.
Appl Environ Microbiol. 2000 Sep;66(9):4145-8. doi: 10.1128/AEM.66.9.4145-4148.2000.
Although the cyanobacterium Anabaena circinalis occurs worldwide, Australian isolates are believed to exclusively possess the saxitoxin group neurotoxins (paralytic shellfish poisons). Identification of A. circinalis in a mixed population is complicated due to limited morphological differences between Anabaena species. Sequence analysis of the DNA-dependent RNA polymerase (rpoC1) gene from 24 Anabaena isolates, including 12 designated A. circinalis, permitted a phylogenetic analysis to be performed. In addition, an A. circinalis-specific PCR was developed and tested successfully on environmental samples.
尽管卷曲鱼腥藻这种蓝藻在全球范围内都有分布，但据信澳大利亚的分离株专门产生石房蛤毒素类神经毒素（麻痹性贝类毒素）。由于鱼腥藻属物种之间形态差异有限，因此在混合群体中鉴定卷曲鱼腥藻很复杂。对包括12株指定为卷曲鱼腥藻的24株鱼腥藻分离株的依赖DNA的RNA聚合酶（rpoC1）基因进行序列分析，从而能够进行系统发育分析。此外，还开发了一种卷曲鱼腥藻特异性PCR，并在环境样本上成功进行了测试。
