Holemans K, Gerber R, O'Brien-Coker I, Mallet A, van Bree R, van Assche F A, Poston L
Department of Obstetrics and Gynecology, Katholieke Universiteit Leuven, 3000, Leuven, Belgium.
Br J Nutr. 2000 Sep;84(3):285-96.
Adult offspring of severely diabetic pregnant rats are insulin resistant and display cardiovascular dysfunction. When pregnant they develop mild hyperglycaemia. Diets high in saturated fat have been implicated in the development of cardiovascular disease and vascular dysfunction. In the present study we have determined vascular function in small mesenteric arteries from offspring of normal (OC) and diabetic (OD) rats fed standard chow and offspring of diabetic rats fed a diet high in saturated fats (OD-HF) from weaning to adulthood, and throughout their subsequent pregnancies. OD rats displayed an increased sensitivity to noradrenaline (P < 0.05) and impaired sensitivity to the endothelium-dependent vasodilator, acetylcholine. The component of acetylcholine-induced relaxation attributable to endothelium-derived hyperpolarizing factor was reduced in OD-HF rats. Pregnant OD rats also demonstrated impaired maximum relaxation to acetylcholine (pregnant OD rats v. pregnant OC rats P < 0.05). In pregnant OD-HF rats noradrenaline sensitivity was enhanced and endothelium-dependent relaxation further reduced (pregnant OD-HF rats v. pregnant OC rats P < 0.001). The isoprostane, 8-epi-prostaglandin F2alpha, a marker of oxidative stress, was increased in pregnant OD rats (pregnant OD rats v. pregnant OC rats P
严重糖尿病妊娠大鼠的成年后代存在胰岛素抵抗并表现出心血管功能障碍。怀孕时，它们会出现轻度高血糖。高饱和脂肪饮食与心血管疾病和血管功能障碍的发生有关。在本研究中，我们测定了从断奶到成年以及随后整个孕期，正常大鼠后代（OC）、糖尿病大鼠后代（OD）喂食标准饲料，以及糖尿病大鼠后代喂食高饱和脂肪饮食（OD-HF）的小肠系膜小动脉的血管功能。OD大鼠对去甲肾上腺素的敏感性增加（P < 0.05），对内皮依赖性血管舒张剂乙酰胆碱的敏感性受损。OD-HF大鼠中，乙酰胆碱诱导的、归因于内皮衍生超极化因子的舒张成分降低。妊娠OD大鼠对乙酰胆碱的最大舒张也受损（妊娠OD大鼠与妊娠OC大鼠相比，P < 0.05）。在妊娠OD-HF大鼠中，去甲肾上腺素敏感性增强，内皮依赖性舒张进一步降低（妊娠OD-HF大鼠与妊娠OC大鼠相比，P < 0.001）。异前列腺素8-表-前列腺素F2α是氧化应激的标志物，在妊娠OD大鼠中升高（妊娠OD大鼠与妊娠OC大鼠相比，P
