Ohsawa M, Narita M, Mizoguchi H, Suzuki T, Tseng L F
Department of Anesthesiology, Medical College of Wisconsin, Medical Education Building, Room 462c, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
Eur J Pharmacol. 2000 Sep 1;403(1-2):81-5. doi: 10.1016/s0014-2999(00)00571-9.
Activation of several protein kinases contributes to the development of hyperalgesia evoked by injuries. The present study was designed to investigate the role of protein kinase C in the spinal cord in thermal hyperalgesia evoked by sciatic nerve ligation or by intraplantar injection of complete Freund's adjuvant. The paw withdrawal latency on the ipsilateral side, but not on the contralateral side, was markedly decreased after sciatic nerve ligation. Intraplantar injection of complete Freund's adjuvant also caused markedly decreases of the paw withdrawal latency. Intrathecal pretreatment with protein kinase C inhibitor calphostin C (100 and 250 ng) attenuated the decrease of the paw withdrawal latency evoked by sciatic nerve ligation. In contrast, the decrease of the paw withdrawal latency evoked by inflammation was only slightly attenuated by intrathecal pretreatment with calphostin C. The results indicate that protein kinase C in the spinal cord is involved in the development of the thermal hyperalgesia evoked by nerve ligation and is much less involved in the thermal hyperalgesia by complete Freund's adjuvant's-induced inflammation.
多种蛋白激酶的激活促成了损伤引起的痛觉过敏的发展。本研究旨在探讨脊髓中蛋白激酶C在坐骨神经结扎或足底注射完全弗氏佐剂所诱发的热痛觉过敏中的作用。坐骨神经结扎后，同侧而非对侧的爪部撤离潜伏期显著缩短。足底注射完全弗氏佐剂也显著缩短了爪部撤离潜伏期。鞘内预先注射蛋白激酶C抑制剂钙泊三醇（100和250 ng）可减轻坐骨神经结扎引起的爪部撤离潜伏期的缩短。相比之下，鞘内预先注射钙泊三醇仅轻微减轻了炎症引起的爪部撤离潜伏期的缩短。结果表明，脊髓中的蛋白激酶C参与了神经结扎诱发的热痛觉过敏的发展，而在完全弗氏佐剂诱导的炎症所致的热痛觉过敏中作用较小。
