酵母细胞中亲和标记生物素转运系统的可逆性

Reversibility of the affinity labelled-biotin transport system in yeast cells.

作者信息

Viswanatha T, Bayer E, Wilchek M

出版信息

Biochim Biophys Acta. 1975 Aug 5;401(1):152-6. doi: 10.1016/0005-2736(75)90349-1.

Abstract

Transport of biotin by Saccharomyces cerevisiae is inhibited by biotynyl p-nitrophenyl ester. Conversion of the inhibited cells to spheroplasts or simple treatment with thiols results in a total restoration of vitamin transport. Biotynyl p-nitrophenyl ester-induced inhibition is not due to an intracellular accumulation of the vitamin and consequent regulation, but appears to be due to specific labelling of the transport system.

摘要

酿酒酵母对生物素的转运受到生物素对硝基苯酯的抑制。将受抑制的细胞转化为原生质体或仅用硫醇处理，可使维生素转运完全恢复。生物素对硝基苯酯诱导的抑制并非由于维生素在细胞内积累并由此产生调节作用，而是似乎由于转运系统的特异性标记。