生物素对硝基苯酯对酵母中生物素转运的不可逆抑制作用。
Irreversible inhibition of biotin transport in yeast by biotinyl-p-nitrophenyl ester.
作者信息
Becker J M, Wilchek M, Katchalski E
出版信息
Proc Natl Acad Sci U S A. 1971 Oct;68(10):2604-7. doi: 10.1073/pnas.68.10.2604.
Abstract
Biotinyl-p-nitrophenyl ester (BNP), an active-ester derivative of biotin, irreversibly inactivates biotin transport in the yeast Saccharomyces cerevisiae. Transport inactivation is progressive with time and occurs at concentrations of the ester as low as 10(-7) M. In the presence of sodium azide, a reagent known to block biotin accumulation in yeast, the derivative is still effective. The specificity of inactivation by the ester is revealed by the following findings: (a) Biotinyl-p-nitroanilide and acetyl-p-nitrophenyl ester do not affect biotin transport; (b) the nitrophenyl ester does not affect the transport of lysine and aspartic acid, or that of L-sorbose; (c) inactivation of biotin transport by the ester is partially prevented when the cells are incubated with it in the presence of relatively high concentrations of biotin.
摘要
生物素对硝基苯酯（BNP）是生物素的一种活性酯衍生物，它能不可逆地使酿酒酵母中的生物素转运失活。转运失活随时间逐渐发生，且在低至10^(-7) M的酯浓度下就会出现。在叠氮化钠（一种已知能阻断酵母中生物素积累的试剂）存在的情况下，该衍生物仍然有效。酯失活的特异性由以下发现揭示：（a）生物素对硝基苯胺和乙酰对硝基苯酯不影响生物素转运；（b）对硝基苯酯不影响赖氨酸、天冬氨酸或L-山梨糖的转运；（c）当细胞在相对高浓度的生物素存在下与酯一起孵育时，酯对生物素转运的失活作用会部分受到抑制。
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