用于诊断副球孢子菌病的聚合酶链反应
PCR for diagnosis of paracoccidioidomycosis.
作者信息
Gomes G M, Cisalpino P S, Taborda C P, de Camargo Z P
机构信息
Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo (UNIFESP), São Paulo, SP, Brazil.
出版信息
J Clin Microbiol. 2000 Sep;38(9):3478-80. doi: 10.1128/JCM.38.9.3478-3480.2000.
Abstract
A PCR assay based on oligonucleotide primers derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis DNA in sputa. In the standardized conditions, it could detect 10 cells/ml of sputum, providing sufficient accuracy to be useful for diagnosis of paracoccidioidomycosis.
摘要
基于编码43000道尔顿（gp43）抗原的基因序列衍生的寡核苷酸引物开发了一种PCR检测方法，用于检测痰液中的巴西副球孢子菌DNA。在标准化条件下，该方法可检测出每毫升痰液中10个细胞，具有足够的准确性，可用于诊断副球孢子菌病。
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本文引用的文献
1
Involvement of the major glycoprotein (gp43) of Paracoccidioides brasiliensis in attachment to macrophages.巴西副球孢子菌主要糖蛋白（gp43）在与巨噬细胞黏附中的作用。
Med Mycol. 1998 Dec;36(6):405-11. doi: 10.1080/02681219880000641.
2
Use of monoclonal antibodies in diagnosis of paracoccidioidomycosis: new strategies for detection of circulating antigens.单克隆抗体在副球孢子菌病诊断中的应用：循环抗原检测的新策略
J Clin Microbiol. 1997 Dec;35(12):3278-83. doi: 10.1128/jcm.35.12.3278-3283.1997.
3
Molecular detection and identification of Paracoccidioides brasiliensis.巴西副球孢子菌的分子检测与鉴定
J Clin Microbiol. 1997 Jul;35(7):1894-6. doi: 10.1128/jcm.35.7.1894-1896.1997.
4
PCR detection of DNA specific for Aspergillus species in serum of patients with invasive aspergillosis.侵袭性曲霉病患者血清中曲霉属特异性DNA的PCR检测
J Clin Microbiol. 1996 Oct;34(10):2464-8. doi: 10.1128/jcm.34.10.2464-2468.1996.
5
Specific components found in circulating immune complexes (CIC) in paracoccidioidomycosis.
J Med Vet Mycol. 1996 Jul-Aug;34(4):273-7. doi: 10.1080/02681219680000461.
6
Paracoccidioides brasiliensis-gp43 used as paracoccidioidin.巴西副球孢子菌-gp43用作副球孢子菌素。
J Med Vet Mycol. 1996 Jun-Jul;34(3):155-61. doi: 10.1080/02681219680000261.
7
Cloning, characterization, and epitope expression of the major diagnostic antigen of Paracoccidioides brasiliensis.巴西副球孢子菌主要诊断抗原的克隆、特性鉴定及表位表达
J Biol Chem. 1996 Feb 23;271(8):4553-60. doi: 10.1074/jbc.271.8.4553.
8
Rapid, polymerase chain reaction-based identification assays for Candida species.基于聚合酶链反应的念珠菌属快速鉴定检测方法。
J Clin Microbiol. 1993 Apr;31(4):904-10. doi: 10.1128/jcm.31.4.904-910.1993.
9
Immunological response to cell-free antigens of Paracoccidioides brasiliensis: relationship with clinical forms of paracoccidioidomycosis.对巴西副球孢子菌无细胞抗原的免疫反应：与副球孢子菌病临床形式的关系。
J Clin Microbiol. 1993 Mar;31(3):671-6. doi: 10.1128/jcm.31.3.671-676.1993.
10
Binding of Paracoccidioides brasiliensis to laminin through surface glycoprotein gp43 leads to enhancement of fungal pathogenesis.巴西副球孢子菌通过表面糖蛋白gp43与层粘连蛋白结合，导致真菌致病性增强。
Infect Immun. 1994 Apr;62(4):1465-9. doi: 10.1128/iai.62.4.1465-1469.1994.
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