利用5'核酸酶分析法对巴西副球孢子菌进行分子鉴定。
Molecular identification of Paracoccidioides brasiliensis by 5' nuclease assay.
作者信息
Semighini Camile P, de Camargo Zoilo P, Puccia Rosana, Goldman Maria Helena S, Goldman Gustavo H
机构信息
Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, São Paulo, Brazil.
出版信息
Diagn Microbiol Infect Dis. 2002 Dec;44(4):383-6. doi: 10.1016/s0732-8893(02)00472-8.
A PCR assay based on the 5' nuclease assay using a fluorescent probe derived from the sequence of the gene coding for the 43,000-Da (gp43) antigen was developed to detect Paracoccidioides brasiliensis. The assay could detect at least 10 copies of this DNA sequence, providing efficient accuracy to be useful for diagnosis of paracoccidioidomycosis.
开发了一种基于5'核酸酶分析的聚合酶链反应（PCR）检测方法，该方法使用一种源自编码43,000道尔顿（gp43）抗原的基因序列的荧光探针来检测巴西副球孢子菌。该检测方法能够检测到至少10个这种DNA序列拷贝，具有高效的准确性，可用于副球孢子菌病的诊断。
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