采用环介导等温扩增法检测痰液中巴西副球孢子菌gp43基因
Detection of Paracoccidioides brasiliensis gp43 gene in sputa by loop-mediated isothermal amplification method.
作者信息
Tatibana B T, Sano A, Uno J, Kamei K, Igarashi T, Mikami Y, Miyaji M, Nishimura K, Itano E N
机构信息
Microbiology Post Graduation Program, State University of Londrina, Londrina, PR, Brazil.
出版信息
J Clin Lab Anal. 2009;23(2):139-43. doi: 10.1002/jcla.20304.
Abstract
The fungus Paracoccidioides brasiliensis is the pathogen of paracoccidioidomycosis (PCM), a systemic mycosis prevalent in Latin America. The loop-mediated isothermal amplification method (LAMP) was used in this study to detect the presence of P. brasiliensis in sputa samples from patients with chronic PCM, suspected PCM, and a negative control. The target P. brasiliensis gp43 gene was amplified in less than 4 hr in 11 of 18 sputa samples tested. The LAMP method had the advantage of speed and simplicity compared with the classic diagnostic methods such as the histopathological test or biological material culture and did not require sophisticated technical apparatus. It would be an important aid in cases where immediate treatment would mean patient survival, especially in immune-suppressed patients.
摘要
巴西副球孢子菌是副球孢子菌病（PCM）的病原体，PCM是一种在拉丁美洲流行的系统性真菌病。本研究采用环介导等温扩增法（LAMP）检测慢性PCM患者、疑似PCM患者痰液样本及阴性对照中巴西副球孢子菌的存在情况。在检测的18份痰液样本中，有11份在不到4小时内扩增出了目标巴西副球孢子菌gp43基因。与组织病理学检查或生物材料培养等经典诊断方法相比，LAMP方法具有速度快和操作简单的优点，且不需要复杂的技术设备。在立即治疗意味着患者存活的情况下，尤其是免疫抑制患者，它将是一项重要的辅助手段。
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