漱口水：一种用于研究细胞因子基因多态性的非侵入性采样方法。
The mouthwash: a non-invasive sampling method to study cytokine gene polymorphisms.
作者信息
Laine M L, Farré M A, Crusius J B, van Winkelhoff A J, Peña A S
机构信息
Academic Centre for Dentistry Amsterdam, Department of Oral Biology, The Netherlands.
出版信息
J Periodontol. 2000 Aug;71(8):1315-8. doi: 10.1902/jop.2000.71.8.1315.
BACKGROUND
We describe a simple, non-invasive mouthwash sampling method for rapid DNA isolation to detect cytokine gene polymorphisms. In the present paper, interleukin- 1beta(IL-1B) and interleukin-1 receptor antagonist (IL-1RN) gene polymorphisms were studied.
METHODS
Two mouthwash samples and blood samples were collected from 11 healthy individuals. The second mouthwash sample was stored for 7 days at room temperature. Polymerase chain reaction amplification was used to identify a bi-allelic polymorphism at position +3953 in the IL-1B gene and a variable number of tandem repeats (VNTR) polymorphism in the IL-1RN gene.
RESULTS
Our results show that the typing of these cytokine gene polymorphisms using DNA isolated from mouthwash samples did not differ from those obtained by a phenol/chloroform isolation method from EDTA anti-coagulated blood. Moreover, reliable results from mouthwash samples were obtained after storage for at least 7 days at room temperature.
CONCLUSIONS
Mouthwash can be the method of choice to study gene polymorphisms in periodontitis and other chronic inflammatory diseases.
背景
我们描述了一种简单、无创的漱口水采样方法，用于快速分离DNA以检测细胞因子基因多态性。在本文中，研究了白细胞介素-1β（IL-1B）和白细胞介素-1受体拮抗剂（IL-1RN）基因多态性。
方法
从11名健康个体中采集两份漱口水样本和血液样本。第二份漱口水样本在室温下保存7天。采用聚合酶链反应扩增来鉴定IL-1B基因中+3953位点的双等位基因多态性以及IL-1RN基因中的可变数目串联重复（VNTR）多态性。
结果
我们的结果表明，使用从漱口水样本中分离的DNA对这些细胞因子基因多态性进行分型，与通过酚/氯仿分离法从EDTA抗凝血液中获得的结果没有差异。此外，漱口水样本在室温下保存至少7天后仍能获得可靠结果。
结论
漱口水可以作为研究牙周炎和其他慢性炎症性疾病基因多态性的首选方法。
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