白细胞介素-1受体拮抗剂基因多态性与种植体周围炎相关。

IL-1RN gene polymorphism is associated with peri-implantitis.

作者信息

Laine Marja L, Leonhardt Asa, Roos-Jansåker Ann-Marie, Peña A Salvador, van Winkelhoff Arie Jan, Winkel Edwin G, Renvert Stefan

机构信息

Department of Oral Microbiology, ACTA Amsterdam, Amsterdam, The Netherlands.

出版信息

Clin Oral Implants Res. 2006 Aug;17(4):380-5. doi: 10.1111/j.1600-0501.2006.01249.x.

DOI:10.1111/j.1600-0501.2006.01249.x

PMID:16907768

Abstract

OBJECTIVES

Interleukin (IL)-1alpha, IL-1beta and their natural specific inhibitor IL-1 receptor antagonist (IL-1ra) play a key role in the regulation of the inflammatory response in periodontal tissues. Polymorphisms in the IL-1 gene cluster have been associated with severe adult periodontitis. We aimed to investigate the IL-1 gene cluster polymorphisms in patients with peri-implantitis.

MATERIAL AND METHODS

The study included 120 North Caucasian individuals. A total of 71 patients (mean age 68 years, 76% smokers) demonstrating peri-implantitis at one or more implants as evidenced by bleeding and/or pus on probing and bone loss amounting to >3 threads on Brånemark implants and 49 controls (mean age 66 years, 45% smokers) with clinical healthy mucosa and no bone loss around the implants were recruited for the study. The titanium implants, ad modum Brånemark, had been in function for at least 2 years. Mouthwash samples were collected and used for genotyping of the bi-allelic polymorphisms IL-1A(-889), IL-1B(+3953), IL-1B(-511) and a variable number of tandem repeat IL-1RN gene polymorphisms using PCR technique.

RESULTS

Significant differences were found in the carriage rate of allele 2 in the IL-1RN gene between peri-implantitis patients and controls (56.5% vs. 33.3%, respectively; odds ratios (OR) 2.6; 95% confidence interval (CI) 1.2-5.6; P=0.015). Logistic regression analysis taking smoking, gender and age into account confirmed the association between the IL-1RN allele 2 carriers and peri-implantitis (OR 3; 95% CI 1.2-7.6; P=0.02).

CONCLUSIONS

Our results provide evidence that IL-1RN gene polymorphism is associated with peri-implantitis and may represent a risk factor for this disease.

摘要

目的

白细胞介素（IL）-1α、IL-1β及其天然特异性抑制剂白细胞介素-1受体拮抗剂（IL-1ra）在牙周组织炎症反应调节中起关键作用。IL-1基因簇多态性与重度成人牙周炎相关。我们旨在研究种植体周围炎患者的IL-1基因簇多态性。

材料与方法

该研究纳入120名北高加索人。共有71例患者（平均年龄68岁，76%为吸烟者），其一个或多个种植体出现种植体周围炎，表现为探诊时出血和/或有脓液，且在Brånemark种植体上骨吸收超过3螺纹；49名对照者（平均年龄66岁，45%为吸烟者），其黏膜临床健康且种植体周围无骨吸收，纳入该研究。采用改良Brånemark型钛种植体，已使用至少2年。收集漱口水样本，采用聚合酶链反应（PCR）技术对双等位基因多态性IL-1A（-889）、IL-1B（+3953）、IL-1B（-511）以及可变数目串联重复IL-1RN基因多态性进行基因分型。

结果

种植体周围炎患者与对照者在IL-1RN基因中2等位基因携带率上存在显著差异（分别为56.5%和33.3%；优势比（OR）2.6；95%置信区间（CI）1.2 - 5.6；P = 0.015）。在考虑吸烟、性别和年龄的逻辑回归分析中，证实IL-1RN 2等位基因携带者与种植体周围炎之间存在关联（OR 3；95% CI 1.2 - 7.6；P = 0.02）。