Bosio K, Avanzini C, D'Avolio A, Ozino O, Savoia D
Department of Clinical and Biological Sciences and Di.VA.P.R.A., University of Torino, Italy.
Lett Appl Microbiol. 2000 Aug;31(2):174-7. doi: 10.1046/j.1365-2672.2000.00785.x.
Propolis, a multifunctional substance used by bees to maintain the safety of their hives, is popular for its therapeutic potential against some micro-organisms. Ethanolic extracts of two propolis specimens, collected from different areas within a region in the north-west of Italy, were examined to evaluate their antimicrobial activity against 46 Streptococcus pyogenes strains. By both agar dilution and agar diffusion methods, the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) were </= 234 microg ml-1, corresponding to a one in 512 dilution of the 12% (w/v) extracts. One of the two propolis samples was more active and this extract was shown to be richer in the flavonoids pinocembrin and galangin using HPLC. Therefore, with a simple microbiological assay technique, in particular the agar dilution method, it was possible to standardize the analysis of propolis samples to identify the quality parameters of this natural product before use for medical treatment.
蜂胶是蜜蜂用于维持蜂巢安全的一种多功能物质，因其对某些微生物具有治疗潜力而广受欢迎。对从意大利西北部一个地区不同地点采集的两种蜂胶样本的乙醇提取物进行了检测，以评估它们对46株化脓性链球菌的抗菌活性。通过琼脂稀释法和琼脂扩散法，最小抑菌浓度（MIC）和最小杀菌浓度（MBC）均≤234微克/毫升，相当于12%（w/v）提取物1/512的稀释液。两个蜂胶样本中的一个活性更强，使用高效液相色谱法（HPLC）显示该提取物中黄酮类化合物松属素和高良姜素的含量更高。因此，通过一种简单的微生物检测技术，特别是琼脂稀释法，可以在将蜂胶样本用于医疗之前对其进行标准化分析，以确定这种天然产物的质量参数。
