Istituto di Scienze Tossicologiche, Igienistiche ed Ambientali, Urbino, Italy.
J Med Food. 2009 Oct;12(5):1050-6. doi: 10.1089/jmf.2008.0173.
The aim of this study was to analyze the antimicrobial activity of two ethanolic extracts of propolis (EEPs) and selected flavonoids against 16 Campylobacter jejuni clinical isolates and several Gram-positive and Gram-negative human pathogens. The antimicrobial activity of EEPs and flavonoids was evaluated by the agar well diffusion method. The EEPs inhibited the growth of C. jejuni, Enterobacter faecalis, and Staphylococcus aureus. The most active flavonoid was galangin, with the highest percentage of sensitivity among C. jejuni strains (68.8%); lower percentages of sensitivity were observed for quercetin (50%). The minimal inhibitory concentrations (MICs) of EEPs and flavonoids for C. jejuni isolates were determined by the agar dilution method. EEPs showed MIC values of 0.3125-0.156 mg/mL for all C. jejuni strains; galangin and quercetin gave MICs ranging from 0.250 to 0.125 mg/mL. Thus propolis preparations could be used as support to traditional therapy for Campylobacter infection, especially when the antibiotic agents show no activity against this microorganism.
本研究旨在分析两种蜂胶乙醇提取物 (EEP) 和几种选定的类黄酮对 16 株空肠弯曲菌临床分离株和一些革兰氏阳性和革兰氏阴性人类病原体的抗菌活性。采用琼脂孔扩散法评估 EEP 和类黄酮的抗菌活性。EEP 抑制空肠弯曲菌、粪肠球菌和金黄色葡萄球菌的生长。最具活性的类黄酮是柽柳素，对空肠弯曲菌菌株的敏感性最高（68.8%）；对槲皮素的敏感性较低（50%）。琼脂稀释法测定了 EEP 和类黄酮对空肠弯曲菌分离株的最小抑菌浓度 (MIC)。EEP 对所有空肠弯曲菌菌株的 MIC 值为 0.3125-0.156mg/mL；柽柳素和槲皮素的 MIC 值范围为 0.250-0.125mg/mL。因此，蜂胶制剂可作为对抗弯曲菌感染的传统疗法的支持，特别是当抗生素对这种微生物没有活性时。
