通过长链反转录聚合酶链反应（long RT-PCR）和限制性片段长度多态性（RFLP）分析对欧洲猪繁殖与呼吸综合征病毒的开放阅读框1进行分析。
Analysis of ORF 1 in European porcine reproductive and respiratory syndrome virus by long RT-PCR and restriction fragment length polymorphism (RFLP) analysis.
作者信息
Nielsen H S, Storgaard T, Oleksiewicz M B
机构信息
Danish Veterinary Institute for Virus Research, Ministry of Agriculture and Fisheries, Lindholm, DK-4771 Kalvehave, Denmark.
出版信息
Vet Microbiol. 2000 Oct 1;76(3):221-8. doi: 10.1016/s0378-1135(00)00258-3.
A rapid method was developed for partial characterization of the replicase-encoding open reading frame 1 (ORF 1) of porcine reproductive and respiratory syndrome virus (PRRSV). It comprised long RT-PCR amplification of 11.1 kb (94%) of ORF 1, followed by restriction fragment length polymorphism analysis. The method was used to compare ORF 1 sequences of two divergent European-type PRRSV strains. Our results indicated that the structural and replicase parts of these two strains had evolved at overall similar rates.
开发了一种快速方法，用于对猪繁殖与呼吸综合征病毒（PRRSV）的复制酶编码开放阅读框1（ORF 1）进行部分特征分析。该方法包括对ORF 1的11.1 kb（94%）进行长RT-PCR扩增，随后进行限制性片段长度多态性分析。该方法用于比较两种不同欧洲型PRRSV毒株的ORF 1序列。我们的结果表明，这两种毒株的结构和复制酶部分总体上以相似的速率进化。
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