由宿主和R因子控制的酶甲基化的噬菌体脱氧核糖核酸序列分析。
Analysis of bacteriophage deoxyribonucleic acid sequences methylated by host- and R-factor-controlled enzymes.
作者信息
May M S, Hattman S
出版信息
J Bacteriol. 1975 Aug;123(2):768-70. doi: 10.1128/jb.123.2.768-770.1975.
Abstract
Phages lambda and fd were propagated in Escherichia coli strains that have either host K-12 or the N-3 R-factor deoxyribonucleic acid-cytosine methylase activity. Pyrimidine tracts containing 3H-labeled 5-methylcytosine (MeC) were analyzed; in all cases, the major methylated sequence was 5' ... C-MeC-T ... 3'.
摘要
噬菌体λ和fd在具有宿主K-12或N-3 R因子脱氧核糖核酸-胞嘧啶甲基化酶活性的大肠杆菌菌株中繁殖。分析了含有3H标记的5-甲基胞嘧啶（MeC）的嘧啶序列；在所有情况下，主要的甲基化序列是5'...C-MeC-T...3'。
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