大肠杆菌K-12 mec+脱氧核糖核酸-胞嘧啶甲基化酶的部分纯化：体外甲基化完全保护噬菌体λ脱氧核糖核酸不被R-EcoRII切割。
Partial purification of the Escherichia coli K-12 mec+ deoxyribonucleic acid-cytosine methylase: in vitro methylation completely protects bacteriophage lambda deoxyribonucleic acid against cleavage by R-EcoRII.
作者信息
Hattman S
出版信息
J Bacteriol. 1977 Mar;129(3):1330-4. doi: 10.1128/jb.129.3.1330-1334.1977.
Abstract
A procedure is described for the partial purification of the deoxyribonucleic acid (DNA)-cytosine methylases controlled by the RII plasmid and by the Escherichia coli mec+ gene. The two enzymes exhibit similar but distinct chromatographic behavior on diethylaminoethyl-cellulose and phosphocellulose. Preliminary studies on the two methylases indicate that they are indistinguishable with respect to their Km for S-adenosylmethionine and their pH (in tris (hydroxymethyl)aminomethane buffer) and NaCl concentration optima. In vitro methylation of various phage lambda DNA substrates by the mec'r RII enzyme modifies the DNA to a form that is completely resistant to double-stranded cleavage by the RII restriction endonuclease (R-EcoRII). These results are consistent with our earlier proposal that the mec8ethylase recognizes RII host specificity sites.
摘要
本文描述了一种用于部分纯化由RII质粒和大肠杆菌mec⁺基因控制的脱氧核糖核酸（DNA）-胞嘧啶甲基化酶的方法。这两种酶在二乙氨基乙基纤维素和磷酸纤维素上表现出相似但不同的色谱行为。对这两种甲基化酶的初步研究表明，它们在对S-腺苷甲硫氨酸的Km值、pH值（在三（羟甲基）氨基甲烷缓冲液中）以及NaCl浓度最适值方面没有区别。mec⁺RII酶对各种噬菌体λ DNA底物的体外甲基化将DNA修饰成一种对RII限制性内切酶（R-EcoRII）的双链切割完全抗性的形式。这些结果与我们早期提出的mec⁺甲基化酶识别RII宿主特异性位点的提议一致。
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Partial purification of the Escherichia coli K-12 mec+ deoxyribonucleic acid-cytosine methylase: in vitro methylation completely protects bacteriophage lambda deoxyribonucleic acid against cleavage by R-EcoRII.大肠杆菌K-12 mec+脱氧核糖核酸-胞嘧啶甲基化酶的部分纯化：体外甲基化完全保护噬菌体λ脱氧核糖核酸不被R-EcoRII切割。
J Bacteriol. 1977 Mar;129(3):1330-4. doi: 10.1128/jb.129.3.1330-1334.1977.
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DNA methylases of Escherichia coli K12. Evidence for changes in their state of association following purification.大肠杆菌K12的DNA甲基化酶。纯化后其结合状态变化的证据。
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Methylation of cytosine residues in DNA controlled by a drug resistance factor (host-induced modification-R factors-N 6 -methyladenine-5-methylcytosine).由耐药因子（宿主诱导修饰-R因子-N6-甲基腺嘌呤-5-甲基胞嘧啶）控制的DNA中胞嘧啶残基的甲基化。
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Mutants of the N-3 R-factor conditionally defective in hspII modification and deoxyribonucleic acid-cytosine methylase activity.在hspII修饰和脱氧核糖核酸 - 胞嘧啶甲基化酶活性方面存在条件性缺陷的N - 3 R因子突变体。
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Isolation of a mutant of Escherichia coli defective in cytosine-specific deoxyribonucleic acid methylase activity and in partial protection of bacteriophage lambda against restriction by cells containing the N-3 drug-resistance factor.分离出一株大肠杆菌突变体，该突变体在胞嘧啶特异性脱氧核糖核酸甲基化酶活性方面存在缺陷，并且在部分程度上不能保护λ噬菌体免受含有N-3耐药因子的细胞的限制作用。
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Nat New Biol. 1973 Jul 11;244(132):40-3. doi: 10.1038/newbio244040a0.
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