Foley Joseph W, Zhu Shirley X, West Robert B
Department of Pathology, Stanford University School of Medicine, Stanford, California, USA.
bioRxiv. 2023 Jul 17:2023.01.13.523849. doi: 10.1101/2023.01.13.523849.
Current methods for profiling DNA methylation require costly reagents, sequencing, or labor time. We introduce FML-seq, a sequencing library protocol that greatly reduces all these costs. Relative to other techniques tested on the same human cell lines, FML-seq produces similar measurements of absolute and differential cytosine methylation at a fraction of the price. FML-seq enables inexpensive, high-throughput experimental designs for large-scale epigenetics research projects.
目前用于分析DNA甲基化的方法需要昂贵的试剂、测序或耗费大量人力时间。我们引入了FML-seq，这是一种测序文库方案，能大幅降低所有这些成本。与在相同人类细胞系上测试的其他技术相比，FML-seq能以极低的成本产生类似的绝对和差异胞嘧啶甲基化测量结果。FML-seq为大规模表观遗传学研究项目提供了经济高效的高通量实验设计方案。
